Identification of AOSC-binding proteins in neurons

Ming Liu; Qin Nie; Xianliang Xin; Meiyu Geng

doi:10.1007/s00343-008-0394-8

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Identification of AOSC-binding proteins in neurons

Updated：2023-05-16

- Identification of AOSC-binding proteins in neurons
- Journal of Oceanology and Limnology Vol. 26, Issue 4, Pages: 394-399(2008)
- Affiliations：
  
  1. Department of Molecular Pharmacology School of Medicine and Pharmacy Ocean University of China
  2. Division of Anti-tumor Pharmacology State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-008-0394-8
  CLC：
- Online First：23 January 2009，
  
  Published：2008
- Accepted：
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Liu, M., Nie, Q., Xin, X. et al. Identification of AOSC-binding proteins in neurons., Chin. J. Ocean. Limnol. 26, 394–399 (2008). https://doi.org/10.1007/s00343-008-0394-8
DOI：

Liu, M., Nie, Q., Xin, X. et al. Identification of AOSC-binding proteins in neurons., Chin. J. Ocean. Limnol. 26, 394–399 (2008). https://doi.org/10.1007/s00343-008-0394-8 DOI：

摘要

Abstract

Acidic oligosaccharide sugar chain (AOSC)

a D-mannuronic acid oligosaccharide

derived from brown algae polysaccharide

has been completed Phase I clinical trial in China as an anti-Alzheimer’s Disease (AD) drug candidate. The identification of AOSC-binding protein(s) in neurons is very important for understanding its action mechanism. To determine the binding protein(s) of AOSC in neurons mediating its anti-AD activities

confocal microscopy

affinity chromatography

and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis were used. Confocal microscopy analysis shows that AOSC binds to SH-SY5Y cells in concentration-

time-

and temperature-dependent fashions. The AOSC binding proteins were purified by affinity chromatography and identified by LC-MS/MS analysis. The results showed that there are 349 proteins binding AOSC

including clathrin

adaptor protein-2 (AP-2) and amyloid precursor protein (APP). These results suggest that the binding/entrance of AOSC to neurons is probably responsible for anti-AD activities.

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references

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