Preparation by enzymolysis and bioactivity of iron complex of fish protein hydrolysate (Fe-FPH) from low value fish

Shanggui Deng; Jiancong Huo; Chao Xie

doi:10.1007/s00343-008-0300-4

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Preparation by enzymolysis and bioactivity of iron complex of fish protein hydrolysate (Fe-FPH) from low value fish

Updated：2023-05-16

- Preparation by enzymolysis and bioactivity of iron complex of fish protein hydrolysate (Fe-FPH) from low value fish
- Journal of Oceanology and Limnology Vol. 26, Issue 3, Pages: 300-306(2008)
- Affiliations：
  
  College of Food and Pharmacology Zhejiang Ocean University
- Author bio：
- Funds：
- DOI：10.1007/s00343-008-0300-4
  CLC：
- Online First：20 August 2008，
  
  Published：2008
- Accepted：
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Deng, S., Huo, J. & Xie, C. Preparation by enzymolysis and bioactivity of iron complex of fish protein hydrolysate (Fe-FPH) from low value fish., Chin. J. Ocean. Limnol. 26, 300–306 (2008). https://doi.org/10.1007/s00343-008-0300-4
DOI：

Deng, S., Huo, J. & Xie, C. Preparation by enzymolysis and bioactivity of iron complex of fish protein hydrolysate (Fe-FPH) from low value fish., Chin. J. Ocean. Limnol. 26, 300–306 (2008). https://doi.org/10.1007/s00343-008-0300-4 DOI：

摘要

Abstract

Preparation of Fe2+ chelate of fish protein hydrolysate (Fe-FPH) obtained from low value fish proteins was introduced and its bioactivity was studied by compound enzymolysis. The optimum conditions for hydrolysate chelating Fe2+ are DH (degree of hydrolysis) at 5%

pH 7.0

20°C and 15 min chelating time for FM (material not being defatted). Four types of Fe-FPH including CA (deposit after chelating)

CB (deposit in 50% of absolute ethanol solution)

CC (suspended deposit in 80% of absolute ethanol solution)

and CD (bottom deposit in 80% of absolute ethanol solution) were fractionated with absolute ethanol from FM. Structural analysis through infra-red spectrum revealed that Fe2+ was combined strongly with amino-group and carboxyl-group in each chelate and each Fe2+ could form two five-member ring structures. All of the four chelates were shown more significant antioxidative activity and can be used as natural hydrophobic and hydrophilic antioxidant. Among all the chelates

the CB possesses the most effective antioxidative activity at 92% as high as that of a-tocopherol. Among all Fe-FPHs

only CD showed the most effective antibacterial activity against Escherichia coli

Staphylococcus aureus

Salmonella typhi

and Bacillus subtilis and can be used as natural antibacterial. It provides a more effective way for utilization of low value fish proteins and key information of Fe-FPH as additive in food industry.

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references

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