Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii

Jinxia Wu; Zhangli Hu; Chaogang Wang; Shuangfei Li; Anping Lei

doi:10.1007/s00343-008-0242-x

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Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii

Updated：2023-05-16

- Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii
- Journal of Oceanology and Limnology Vol. 26, Issue 3, Pages: 242-247(2008)
- Affiliations：
  
  Institute of Ecology and Environment College of Life Sciences Shenzhen University
- Author bio：
- Funds：
- DOI：10.1007/s00343-008-0242-x
  CLC：
- Online First：20 August 2008，
  
  Published：2008
- Accepted：
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Wu, J., Hu, Z., Wang, C. et al. Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii, ., Chin. J. Ocean. Limnol. 26, 242–247 (2008). https://doi.org/10.1007/s00343-008-0242-x
DOI：

Wu, J., Hu, Z., Wang, C. et al. Efficient expression of green fluorescent protein (GFP) mediated by a chimeric promoter in Chlamydomonas reinhardtii, ., Chin. J. Ocean. Limnol. 26, 242–247 (2008). https://doi.org/10.1007/s00343-008-0242-x DOI：

摘要

Abstract

To improve the expression efficiency of exogenous genes in Chlamydomonas reinhardtii

a high efficient expression vector was constructed. Green fluorescent protein (GFP) was expressed in C. reinhardtii under the control of promoters: RBCS2 and HSP70A-RBCS2. Efficiency of transformation and expression were compared between two transgenic algae: RBCS2 mediated strain Tran-I and HSP70A-RBCS2 mediated strain Tran-II. Results show that HSP70A-RBCS2 could improve greatly the transformation efficiency by approximately eightfold of RBCS2

and the expression efficiency of GFP in Tran-II was at least double of that in Tran-I. In addition

a threefold increase of GFP in Tran-II was induced by heat shock at 40°C. All of the results demonstrated that HSP70A-RBCS2 was more efficient than RBCS2 in expressing exogenous gene in C. reinhardtii.

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references

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