Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri

Yang Zhang; Xiaojun Zhang; Chantel F. Scheuring; Hongbin Zhang; Fuhua Li; Jianhai Xiang

doi:10.1007/s00343-008-0215-0

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Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri

Updated：2023-05-16

- Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri
- Journal of Oceanology and Limnology Vol. 26, Issue 2, Pages: 215-218(2008)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Graduate School Chinese Academy of Sciences
  3. Department of Soil & Crop Sciences Texas A&M University
- Author bio：
- Funds：
- DOI：10.1007/s00343-008-0215-0
  CLC：
- Online First：28 May 2008，
  
  Published：2008
- Accepted：
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Zhang, Y., Zhang, X., Scheuring, C.F. et al. Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri, ., Chin. J. Ocean. Limnol. 26, 215–218 (2008). https://doi.org/10.1007/s00343-008-0215-0
DOI：

Zhang, Y., Zhang, X., Scheuring, C.F. et al. Construction of bacterial artificial chromosome libraries for Zhikong Scallop Chlamys farreri, ., Chin. J. Ocean. Limnol. 26, 215–218 (2008). https://doi.org/10.1007/s00343-008-0215-0 DOI：

摘要

Abstract

Two Large-insert genomic bacterial artificial chromosome (BAC) libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I

respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb

providing a coverage of 5.3 haploid genome equivalents. Similarly

the Mbo I library with an average insert of 145 kb and no insert-empty clones

thus providing a genome coverage of 1.1 haploid genome equivalents.

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references

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