Purification and characterization of vitellin in mature ovary of marine crab Charybdis japonica

Song Xie; Chengxiu Guo; Fengsong Liu; Xiaohui Liang

doi:10.1007/s00343-008-0162-9

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Purification and characterization of vitellin in mature ovary of marine crab Charybdis japonica

Updated：2023-05-16

- Purification and characterization of vitellin in mature ovary of marine crab Charybdis japonica
- Journal of Oceanology and Limnology Vol. 26, Issue 2, Pages: 162-165(2008)
- Affiliations：
  
  1. College of Life Sciences Hebei University
  2. Oceans and Fisheries Bureau of Dongying
  3. College of Biotechnology Southern Yangtze University
- Author bio：
- Funds：
- DOI：10.1007/s00343-008-0162-9
  CLC：
- Online First：28 May 2008，
  
  Published：2008
- Accepted：
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Xie, S., Guo, C., Liu, F. et al. Purification and characterization of vitellin in mature ovary of marine crab Charybdis japonica, ., Chin. J. Ocean. Limnol. 26, 162–165 (2008). https://doi.org/10.1007/s00343-008-0162-9
DOI：

Xie, S., Guo, C., Liu, F. et al. Purification and characterization of vitellin in mature ovary of marine crab Charybdis japonica, ., Chin. J. Ocean. Limnol. 26, 162–165 (2008). https://doi.org/10.1007/s00343-008-0162-9 DOI：

摘要

Abstract

Vitellin of mature female marine crab Charybdis japonica was purified by high-performance liquid chromatography (HPLC

DEAE-cellulose-16 anion exchange column). The apparent molecular mass of the vitellin is 546 ku based on the data of native-PAGE. Under denatured condition (SDS-PAGE)

it was found that vitellin was composed of four polypeptides each at 120

100

and 55 ku. One disulfide bond was detected in the binding of polypeptide subunits. The purified vitellin

contained 4.47% phosphor and 10.6% polysaccharides

and was identified as glyco-lipo-carotenoprotein

according to the PAGE staining data. The purified vitellin can be used as antigen to raise polyclonal antisera in further application.

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Keywords

references

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