Strain H2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation

Yanhong Sun; Jianguo Liu; Xiaoli Zhang; Wei Lin

doi:10.1007/s00343-008-0152-y

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Strain H2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation

Updated：2023-05-16

- Strain H2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation
- Journal of Oceanology and Limnology Vol. 26, Issue 2, Pages: 152-156(2008)
- Affiliations：
  
  1. R &D Center of Marine Biotechnology Institute of Oceanology Chinese Academy of Sciences
  2. Graduate School of the Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-008-0152-y
  CLC：
- Online First：28 May 2008，
  
  Published：2008
- Accepted：
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Sun, Y., Liu, J., Zhang, X. et al. Strain H2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation., Chin. J. Ocean. Limnol. 26, 152–156 (2008). https://doi.org/10.1007/s00343-008-0152-y
DOI：

Sun, Y., Liu, J., Zhang, X. et al. Strain H2-419-4 of Haematococcus pluvialis induced by ethyl methanesulphonate and ultraviolet radiation., Chin. J. Ocean. Limnol. 26, 152–156 (2008). https://doi.org/10.1007/s00343-008-0152-y DOI：

摘要

Abstract

Two strains H2-410 and H2-419 were obtained from the chemically mutated survivors of wild Haematococcus pluvialis 2 by using ethyl methanesulphonate (EMS). Strains H2-410 and H2-419 showed a fast cell growth with 13% and 20% increase in biomass compared to wild type

respectively. Then H2-419-4

a fast cell growth and high astaxanthin accumulation strain

was obtained by exposing the strain H2-419 to ultraviolet radiation (UV) further. The total biomass

the astaxanthin content per cell

astaxanthin production of H2-419-4 showed 68%

28%

and 120% increase compared to wild H. pluvialis 2

respectively. HPLC (High Performance Liquid Chromatography) data showed also an obvious proportional variation of different carotenoid compositions in the extracts of H2-419-4 and the wild type

although no peak of carotenoids appeared or disappeared. Therefore

the main compositions in strain H2-419-4

like its wild one

were free of astaxanthin

monoester

and diester of astaxanthin. The asexual reproduction in survivors after exposed to UV was not synchronous

and different from the normal synchronous asexual reproduction as the mother cells were motile instead of non-motile. Interestingly

some survivors from UV irradiation produced many mini-spores (or gamete?)

the spores moved away from the mother cell gradually 4 or 5 days later. This is quite similar to sexual reproduction described by Elliot in 1934. However

whether this was sexual reproduction remains questionable

as no mating process has been observed.

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references

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