Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus

Wen Luo; Yunlong Zhao; Zhongliang Zhou; Chuanguang An; Qiang Ma

doi:10.1007/s00343-008-0062-z

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Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus

Updated：2023-05-16

- Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus
- Journal of Oceanology and Limnology Vol. 26, Issue 1, Pages: 62-68(2008)
- Affiliations：
  
  1. School of Life Science College of Shaoxing Arts and Sciences
  2. School of Life Science East China Normal University
- Author bio：
- Funds：
- DOI：10.1007/s00343-008-0062-z
  CLC：
- Published：2008
- Accepted：
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Luo, W., Zhao, Y., Zhou, Z. et al. Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus, ., Chin. J. Ocean. Limnol. 26, 62–68 (2008). https://doi.org/10.1007/s00343-008-0062-z
DOI：

Luo, W., Zhao, Y., Zhou, Z. et al. Digestive enzyme activity and mRNA level of trypsin in embryonic redclaw crayfish, Cherax quadricarnatus, ., Chin. J. Ocean. Limnol. 26, 62–68 (2008). https://doi.org/10.1007/s00343-008-0062-z DOI：

摘要

Abstract

The digestive enzyme activity and mRNA level of trypsin during the embryonic development of Cherax quadricarinatus were analyzed using biochemical and Fluorogenic Quantitative PCR (FQ—PCR) methods. The results show that the activities of trypsin and chymotrypsin had two different change patterns. Trypsin specific activity increased rapidly in the early stages of development and still remained high in preparation for the hatch stage. However

chymotrypsin activity peaked in stage 4 of embryonic development and decreased significantly in the last stage. The mRNA level of trypsin was elevated in all stages and two peak values were observed in stages 2 and 5 respectively. The results indicate that trypsin is very important for the utilization of the yolk during embryonic development and for the assimilation of dietary protein for larvae. The gene of trypsin is probably regulated at transcriptional level. The mRNA levels of trypsin can reflect not only trypsin activity

but also the regulatory mechanism for expression of trypsin gene to a certain degree.

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references

Muhlia-Almazán, A., F. L. Garcia-Carreňo, J. A. Sánchez Paz, G. Yepiz-Plascencia and A. B. Peregrino-Uriarte, 2003. Effects of dietary protein on the activity and mRNA level of trypsin in the midgut gland of the white shrimp Penaeus vannamei. Comp. Biochem. Physiol. 128B: 565–577.

Al-Mohanna, S. Y. and J. A. Nott, 1989. Functional cytology of the hepatopancreas of Penaeus semisulcatus (crustacean: decapoda) during the molting cycle. Mar. Biol. 101: 535–544.

Sánchez Paz, A., F. L. Garcia-Carreňo, A. Muhlia-Almazán, N. Y. Hernández-Saavedra and G. Yepiz-Plascencia, 2003. Differential expression of trypsin mRNA in the white shrimp (Penaeus vannamei) midgut gland under starvation conditions. J. Exp. Mar. Biol. Ecol. 292: 1–17.

Babu, D. E., 1987. Observations on the embryonic development and energy source in the crab Xantho bidentatus. Mar. Biol. 95: 123–127.

Bradford, M. M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.

Briggs, R. and G. Cassens, 1996. Accumulation in the oocyte nucleus of a gene product essential for embryonic development beyond gastrula. Proc. Natl. Acad. Sci. USA, 55: 1 103–1 109

Bustamante, E., J. Palomino, A. Amoroso, R. Moreno and C. Barros, 2001. Purification and biochemical characterization of a trypsin-like enzyme present in the sperm of the rock shrimp, Rhynchocinetes typus. Invertber. Reprod. Dev. 39(3): 175–181.

Fernandez-Gimenez, A. V., F. L. Garcia-Carreňo, M. A. Navarrete del Toro and J. L. Fenucci, 2001. Digestive proteinases of red shrimp Pleoticus muelleri (Decapoda, Penaeoidea): partial characterization and relationship with molting. Comp. Biochem. Physiol. 130B: 331–338.

Galgani, F. G., Y. Renyamin and H. J. Ceccaldi, 1984. Identification of digestive proteinases of Penaeus japonicus Bate. Comp. Biothem. Physiol. 78: 355–361.

Galgani, F. G., 1985. Regulation de l’activite des proteases digestives de Penaeus japonicus Bate en relation avec la temperature. J. Exp. Mar. Biol. Ecol. 94: 11–18.

Garcia-Guerrero, M., I. S. Racotta and H. Villarreal, 2003. Variation in lipid, protein and carbohydrate content during the embryonic development of the crayfish C.quadricarinatus (Decapoda: Parastacidae). J. Crust. Biol. 23(1): 1–6.

Hernandez-Cortes, P., L. Cerenius, F. L. Garcia-Carreňo and K. Soderhall, 1999. Trypsin from Pacifastacus leniusculus hepatopancreas: purification and cDNA cloning of the synthesized zymogen. Biol. Chem. 380(4): 499–501.

Hernandez, P., W. Quadros, A. Navarrete, G. Portillo, G. Colado and F. L. Garcia-Carreňo, 1999. Rate of ingestion and proteolytic activity in digestive system during continuous feeding of juvenile shrimps. J. Appl. Aquacult. 9: 35–45.

Jones, C. M. and I. M. Ruscoe, 2002. Biological and aquaculture characteristics of five stocks of redclaw, Cherax quadricarinatus (von Martens) (Decapoda: Parastacidae) from northern Queensland, Australia Freshwater Crayfish. Proceedings of the Thirteenth Symposium of the International Association of Astacology. 115–135.

Klein, B., G. Le Moullac, D. Sellos and A. van Wormhoudt, 1996. Molecular cloning and sequencing of trypsin cDNAs from Penaeus vannamei (Crustacean, Decapoda): used in assessing gene expression during the moult cycle. Int. J. Biochem. Cell Biol. 28(5): 551–563.

Koshio, S., S. Teshima, A. Kanazawa and T. Watase, 1993. The effect of dietary protein content on growth, digestion efficiency and nitrogen excretion of juvenile kuruma prawns, Penaeus japonicus. Aquaculture. 113: 101–114.

Le Moullac, G. and A. van Wormhoudt, 1994. Adaptation of digestive enzymes to dietary protein, carbohydrate and fiber levels and influence of protein and carbohydrate quality in Penaeus vannamei larvae (crustacean, decapoda). Aquat. Living Resour. 7: 203–210.

Lee, P. G., L. L. Smith and A. L. Lawrence, 1984. Digestive protease of Penaeus vannamei Boone: relationship between enzyme activity, size and diet. Aquaculture 42: 225–239.

Lemos, D., M. P. Hernandez-Cortes, A. Navarrete, F. L. Garcia-Carreňo and V. N. Phan, 1999. Ontogenetic variation in digestive proteinase activity of larvae and postlarvae of the pink shrimp Farfantepenaeus paulensis (Crustacean: Decapoda: Penaeidae). Mar. Biol. 135: 653–662.

Lemos, D., J. M. Ezquerra and F. L. Garcia-Carreňo, 2000. Protein digestion in penaeid shrimp: digestive proteinases, proteinase inhibitors and feeding digestibility. Aquaculture 186: 89–105.

Lovett, D. L. and D. L. Felder, 1990. Ontogemc change m enzyme activities of larval and postlarval white shrimp Prnurus setiferus (Crustacean. Decapoda). Biol. Bull. 178: 146–159.

Luo, W., Q. Wan, Y. L. Zhao, Z. B. Yang and M. F. Zhang, 2004. Analysis on the contents of protein and amino acids in Cherax quadricarinatus during different embryonic development stages. Journal of East China Normal University (Nature Science) 1: 88–92. (in Chinese).

Meng, F. L., Y. L. Zhao, L. Q. Chen, Z. M. Gu, G. X. Xu and Q. W. Liu, 2001. Embryonic Development of Redclaw Crayfish Cherax quadricarinatus: II. Development of Digestive System. Zool. Res. (in Chinese). 22(5): 383–386.

Muhlia-Almazán, A., F. L. Garcia-Carreňo, J. A. Sánchez Paz, G. Yepiz-Plascencia and A.B. Peregrino-Uriarte, 2003. Effects of dietary protein on the activity and mRNA level of trypsin in the midgut gland of the white shrimp Penaeus vannamei. Comp. Biochem. Physiol. 135B: 373–383.

Petersen, S. and K. Anger, 1997. Chemical and physiological changes during the embryonic development of the spider crab Hyas arenus (Decapoda: Majidae). Comp. Biochem. Physiol. 117b(2): 299–306.

Rodrıguez, A., L. Le Vay, G. Mourente and D. A. Jone, 1994. Biochemical composition and digestive enzyme activity in larvae and postlarvae of Penaeus japonicus during herbivorous and carnivorous feeding. Mar. Biol. 118: 45–51.

Sanchez Paz, J. A., 2001. Adaptive regulation of trypsin mRNA of the white shrimp (Penaeus vannamei) hepatopancreas under starvation. Cent. de Investigaciones Biologicas del Noroeste, La Paz, Baja California Sur, (Mexico). 62.

Sanchez Paz, J. A., F. L. Garcia-Carreňo., A. Muhlia-Almazán, N.Y. Hernández-Saavedra and G. Yepiz-Plascencia, 2003. Differential expression of trypsin mRNA in the white shrimp (Penaeus vannamei) midgut gland under starvation conditions. Journal of Experimental Marine Biology and Ecology 292: 1–17.

Schmittgen, T. D. and B. A. Zakrajsek, 2000. Effect of experimental treatment on housekeeping gene expression: validation by real-time, quantitative RT-PCR. J. Biochem. Biophys. 46: 69–81.

Van Wormhoudt, A., 1973. Variations des proteases, des amylases et des protéines solubles au cours de dé veloppement larvaire chez Palemon serratus. Mar Biol. 19: 245–248.

Yeh, H. and D. Rouse, 1994. Indoor spawning and eggs development of the red claw crayfish Cherax quadricarinatus. J. World Aquac. Soc. 25: 297–302.

Zhao, Y. L., F. L. Meng, L. Q. Chen, Z. M. Gu, G. X. Xu and Q. W. Liu, 2000. Effects of Different Gradient Temperatures on Embryonic Development of the Cherax quadricarinatus (Crustacea, Decapoda). J. Lake Sci. 12(1): 59–62. (in Chinese).

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