Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)

Jindong Zan; Xiuqin Sun; Zhiwen Zhang; Lingyun Qu; Jinxing Zhang

doi:10.1007/s00343-007-0418-9

Your Location：

Home >

Browse articles >

Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)

Updated：2023-05-16

- Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)
- Journal of Oceanology and Limnology Vol. 25, Issue 4, Pages: 418-422(2007)
- Affiliations：
  
  First institute of Oceanography SOA
- Author bio：
- Funds：
- DOI：10.1007/s00343-007-0418-9
  CLC：
- Published：2007
- Accepted：
Scan QR Code
Zan, J., Sun, X., Zhang, Z. et al. Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)., Chin. J. Ocean. Limnol. 25, 418–422 (2007). https://doi.org/10.1007/s00343-007-0418-9
DOI：

Zan, J., Sun, X., Zhang, Z. et al. Application of quantitative PCR method in detection of Lymphocystis disease virus China (LCDV-cn) in Japanese flounder (Paralichthys olivaceus)., Chin. J. Ocean. Limnol. 25, 418–422 (2007). https://doi.org/10.1007/s00343-007-0418-9 DOI：

摘要

Abstract

Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China (LCDV-cn)

which has a wide range of hosts. Based on competitive quantitative PCR technology

we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 106 virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 104 virions/ml. No virus was found in the negative samples of flounder.

关键词

Keywords

references

Gilliland, G., S. Perrin, K. Blanchard and H. F. Bunn, 1990. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Pron. Natl, Acad. Sci. USA 87: 2 725–2 729.

Liu, Y. K, X. Q. Sun, J. Huang and J. X. Zhang, 2002 The diagnostic method of PCR for Lymphocystis Disease of Cultured Paralichthys olivaceus. High Tchnol. Lett. 11: 18. (in Chinese)

Ozawa, T., M. Anaka, S. Ikebe, K. Ohno, T. Kondo and Y. Mizuno, 1990. Quantitative determination of deleted mitochondrial DNA relative to normal DNA in parkinsonian striatum by a kinetic PCR analysis. Biochem. Biophys, Res. Commun. 172: 483–448.

Seibel, P., O. Mell, A. Hannemann, J. Muller-Hocker and B. Kadenbach, 1991. A method for quantitative analysis of detected mitochondrial DNA by PCR in small tissue samples. Methods Mol. Cell. Biol. 2: 147–153.

Sun, X. Q., L. Qu and J. X. Zhang, 2000. Pathogenicity and immunogenicity of lymphocystis virus of Japanese flounder (Paralichthys olivaceus). High Tchnol. Lett. 9: 19. (in Chinese)

Silvia Diviacco, Paolo Norio, Lorena Zentilin, Stefano Menzo, Massimo Clementi, Giuseppe Biamonti, Silvano Riva, Arturo Falaschi and Mauro Giacca. 1992 A novel procedure for quantitative polymerase chain reaction by coamplification of competitive templates. Gene, 15, 122(2): 313–320

Tidona, C. A. and G. Darai, 1999. Lymphocystis disease virus (Iridoviridae). In: A. Granofl and R. G. Webster (ed.), Encyclopedia of Virology. Academic Press. New York. p. 908–911.

Xu L. M., W. Wang and F. Yang, 2001. Research of Quantitative PCR Detection Technology of White Spot Bacilliform VIrus(WSBV). High Tech. Letter. 1: 14–16.

Xu, L. M. and F. Yang, 2001. Research of the relationship of White Spot Bacilliform Virus Infective Level and Disease Outbreak in Cultured Shrimp by quantitative PCR. p. 12, 9–11.

Wang, A. M., M. V. Doyle and D. F. Mark, 1989. Quantitation of mRNA by the polymerase chain reaction. Pron. Natl, Acad. Sci. USA 86: 9 717–9 721.

Zhang, Q. Y., X. Feng, X. Jian, Z. Q. Li and J. F. Gui, 2004. Complete genome sequence of Lymphocystis disease virus isolated from China. Journal of Virology 78: 6 982–6 994.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Proteomic aspects of infection by lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)

Wall Corrosion Morphology Prediction of Hall ThrustersUsing Semi-Empirical Method

Research on Aging Characterization of Solid Propellantsby Electro-Mechanical Impedance

Method of Point Selection for Fitting Ignition Probability Curve Based on Maximum Likelihood Estimation

Research on Unsteady Characteristics ofUnderwater Supersonic Gas Jet

Related Author

Mingqing Xing

Xiuqin Sun

Fengrong Zheng

Lingyun Qu

Xuguang Hong

Suqi Wu

Minggang Zheng

Jinxing Zhang

Related Institution

College of Marine Life Sciences Ocean University of China

Department of Clinical Laboratory Qingdao Municipal Hospital

The First Institute of Oceanography SOA

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰