Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins

Hongming Ma; Kangsen Mai; Zhiguo Liufu; Wei Xu

doi:10.1007/s00343-007-0304-5

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Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins

Updated：2023-05-16

- Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins
- Journal of Oceanology and Limnology Vol. 25, Issue 3, Pages: 304-309(2007)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Life Science and Technology Division Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-007-0304-5
  CLC：
- Published：2007
- Accepted：
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Ma, H., Mai, K., Liufu, Z. et al. Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins., Chin. J. Ocean. Limnol. 25, 304–309 (2007). https://doi.org/10.1007/s00343-007-0304-5
DOI：

Ma, H., Mai, K., Liufu, Z. et al. Cloning and characterization of an actin gene of Chlamys farreri and the phylogenetic analysis of mollusk actins., Chin. J. Ocean. Limnol. 25, 304–309 (2007). https://doi.org/10.1007/s00343-007-0304-5 DOI：

摘要

Abstract

An actingene (CfACTI) was cloned by using RT-PCR

3’ and 5’RACE from hemocytes of the sea scallop Chlamys farreri. The full length of the transcript is 1 535 bp

which contains a long 3’ un-translated region of 436bp and 59bp of a 5’ un-translated sequence. The open reading frame encodes a polypeptide of 376 amino acids. Sequence comparisons indicated that CfACTI is more closely related to vertebrate cytoplasmic actins than muscle types. Phylogenetic analysis showed that molluscan actins could be generally divided into two categories: muscle and cytoplasmic

although both are similar to vertebrate cytoplasmic actins. It was also inferred that different isotypes existed in muscle or cytoplasma in mollusks. The genomic sequence of CfACTI was cloned and sequenced. Only one intron was detected: it was located between codons 42 and 43 and different from vertebrate actin genes.

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references

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