Antioxidation and ATPase activity in the gill of mud crab Scylla serrata under cold stress

Xianghui Kong; Guizhong Wang; Shaojing Li

doi:10.1007/s00343-007-0221-7

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Antioxidation and ATPase activity in the gill of mud crab Scylla serrata under cold stress

Updated：2023-05-16

- Antioxidation and ATPase activity in the gill of mud crab Scylla serrata under cold stress
- Journal of Oceanology and Limnology Vol. 25, Issue 2, Pages: 221-226(2007)
- Affiliations：
  
  1. Department of Oceanography State Key Laboratory for Marine Environmental Science Xiamen University
  2. College of Life Science Henan Normal University
- Author bio：
- Funds：
- DOI：10.1007/s00343-007-0221-7
  CLC：
- Published：2007
- Accepted：
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Kong, X., Wang, G. & Li, S. Antioxidation and ATPase activity in the gill of mud crab Scylla serrata under cold stress., Chin. J. Ocean. Limnol. 25, 221–226 (2007). https://doi.org/10.1007/s00343-007-0221-7
DOI：

Kong, X., Wang, G. & Li, S. Antioxidation and ATPase activity in the gill of mud crab Scylla serrata under cold stress., Chin. J. Ocean. Limnol. 25, 221–226 (2007). https://doi.org/10.1007/s00343-007-0221-7 DOI：

摘要

Abstract

Mud crab (Scylla serrata) is an important commercial crustacean in China. An experiment was designed to study the effect of cold stress on S. serrata. After a one-week adaptation at 28°C

the temperature is suddenly reduced to 4°C. The crabs were sampled every 2 h for 10 h and dissected immediately to measure the enzyme activity. The crabs at room temperature (28°C) were used as the control group. The activity of superoxide dismutase (SOD)

catalase (CAT) and glutathione peroxidase (GPX)

the content of malondialdehyde (MDA) and the activity of 4 ATPases (Na+

K+-ATPase; Mg2+-ATPase; Ca2+-ATPase; Ca2+

Mg2+-ATPase) were measured biochemically. In contrast to the control group

the SOD activity increased significantly from 2 to 6 h after the cold stress

and then decreased. The CAT and GPX activities increased in 2 h

and then decreased gradually. The content of MDA increased gradually in 4 h. The activity of Na+

K+-ATPase decreased in 2 h

increased up to the top value at Hour 6

then decreased again. The activities of Mg2+-ATPase

Ca2+-ATPase and Ca2+

Mg2+-ATPase increased significantly in 6 h

insignificantly in any other hours. Under cold stress

the activity of antioxidative enzymes in S. serrata was reduced at first then stabilized

ROS-scavenging weakened

and MDA accumulated gradually in the gill after 6 h. The activity of the 4 ATPases in the crab decreased after 6 h

suggesting that the ability to regulate ion concentration has been paralyzed. Therefore

the maximum period to sustain healthy meat in the crab under cold stress is 6 hours.

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