In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus

Feng Su; Shicui Zhang; Hongyan Li; Huarong Guo

doi:10.1007/s00343-007-0209-3

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In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus

Updated：2023-05-16

- In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus
- Journal of Oceanology and Limnology Vol. 25, Issue 2, Pages: 209-214(2007)
- Affiliations：
  
  1. Department of Bioengineering & Pharmaceutics Qingdao University of Science & Technology
  2. Department of Marine Biology Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-007-0209-3
  CLC：
- Published：2007
- Accepted：
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Su, F., Zhang, S., Li, H. et al. In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus., Chin. J. Ocean. Limnol. 25, 209–214 (2007). https://doi.org/10.1007/s00343-007-0209-3
DOI：

Su, F., Zhang, S., Li, H. et al. In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid to gill cell line of flounder Paralichthy olivaceus., Chin. J. Ocean. Limnol. 25, 209–214 (2007). https://doi.org/10.1007/s00343-007-0209-3 DOI：

摘要

Abstract

In vitro acute cytotoxicity of neonicotinoid insecticide imidacloprid (IMI) to the gill cell line of flounder (FG) that collected in the gill of Paralichthys olivaceus

was examined by 3 widely used endpoint bioassays: NR (neutral red)

MTT (3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide) and TCP (total cell protein). The result shows that the IMI increased at concentrations ≥0.5 μg/ml. The IC50 value of NR. MTT

and TCP was 41.86

38.46

and 39.08 μg/ml

respectively. The ultrastructural observation revealed that the mitochondria of the cells exposed to 60 μg/ml IMI for 48 h were severely damaged

swollen or disrupted

while their nuclei and rough endoplasmic reticulum (RER) remained normal. This would suggest that the mitochondria are probably the primary target of IMI.

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references

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