Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers

Bing Yao; Xiaoli Hu; Zhenmin Bao; Wei Lu; Jingjie Hu

doi:10.1007/s00343-007-0091-z

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Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers

Updated：2023-05-16

- Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers
- Journal of Oceanology and Limnology Vol. 25, Issue 1, Pages: 91-96(2007)
- Affiliations：
  
  Laboratory of Marine Genetics and Breeding (MGB) Division of Life Science and Technology Ocean University of China
- Author bio：
- Funds：
- DOI：10.1007/s00343-007-0091-z
  CLC：
- Published：2007
- Accepted：
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Yao, B., Hu, X., Bao, Z. et al. Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers., Chin. J. Ocean. Limnol. 25, 91–96 (2007). https://doi.org/10.1007/s00343-007-0091-z
DOI：

Yao, B., Hu, X., Bao, Z. et al. Genetic variation in two sea cucumber (Apostichopus japonicus) stocks revealed by ISSR markers., Chin. J. Ocean. Limnol. 25, 91–96 (2007). https://doi.org/10.1007/s00343-007-0091-z DOI：

摘要

Abstract

Sea cucumber Apostichopus japonicus samples were collected in Changdao

Penglai (PL)

27 individuals

and Lingshandao

Qingdao (QD)

30 individuals

in the Shandong Peninsula

China. Ten SSR primers were used to assess the genetic variation and relationship between and within the two stocks. Respectively

for each stock

the percentage of polymorphic bands was 85.2% and 86.9%; the gene diversity was 0.360 5 and 0.342 8; and the Shannon’s information index was 0.515 0 and 0.499 0. At species level

the percentage of polymorphic bands was 92.2%

the total gene diversity was 0.378 9 and the Shannon’s information index was 0.550 4. The coefficient of overall genetic differentiation and the genetic distances between the stocks were also calculated to be 0.073 0 and 0.079 6 using the POPGENE program. Results show that the genetic diversity of the two stocks is still large but the genetic distance between the two stocks is close. A dendrogram was constructed for the 57 individuals from the two stocks

showing that the genetic structure was unitary for PL stock but complex for QD stock.

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references

Chen, D. P., H. S. Shen, Y. P. Ding, J. X. Yang, S. D. Shen and P. Xu, 2004. Using Inter-simple Sequence Repeats (ISSR) technique in two populations of Meretrix meretrix. Journal of Nanjing Normal University 27: 74–77. (in Chinese)

Dinesh, K. R., T. M. Lim, K. L. Chua, W. K. Chan and V. P. Phang. 1993. RAPD analysis: an efficient method of DNA fingerprinting in fishes. Zoology Science 10: 849–854.

Doyle, J. J. and J. L. Doyle, 1990. Isolation of plant DNA from fresh tissue. Focus 12: 13–15.

Felsenstein, K. M. and L. Lewis-Higgins, 1993. Processing of the beta-amyloid precursor protein carrying the familial, Dutch-type and a novel recombinant C-terminal mutation. Neuroscience Letters 152: 185–189.

Gao, Y. M. and J. B. Sun, 2004. The biochemical genetics of Stichopus japonnicus Selenka population. Journal of Dalian Fisheries University 19: 30–34. (in Chinese)

Garcia, D. K. and J. A. H. Benzie, 1995. RAPD markers of potential use in penaeid prawn (Penaeus monodon) breeding programs. Aquaculture 130: 137–144

Godwin, I. D., E. A. B. Aitken and L. W. Smith, 1997. Application of inter-simple sequence repeat (ISSR) markers to plant genetics. Electrophoresis 18: 1 524–1 528.

Gupta, M., Y. S. Chyi, J. Romero-Severson and J. L. Owen, 1994. Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats. Theoretical and Applied Genetics 89: 998–1006.

Hamrick, J. E. and M. J. W. Godt, 1996. Conservation genetics of endemic plant species. In: J.C. Avise and J.E. Hamrick ed., Conservation Genetics: Case Histories from Nature. Chapman and Hall, New York, p. 281–304.

Kanno, M. and A. Kijima, 2002. High genetic variability of isozymes in Japanese sea cucumber Stichopus japonicus. Fisheries Genetics and Breeding Sciences 31: 7–12.

Kanno, M. and A. Kijima, 2003. Genetic differentiation among three color variants of Japanese sea cucumber Stichopus japonicus. Fisheries Science 69: 806–812.

Lewinton, R. C., 1972. The apportionment of human diversity. Evolutionary Biology 6: 381–398.

Liu, P., X. H. Meng, J. Kong, J. Li and W. J. Wang, 2002. RAPD analysis of genetic markers affiliated with disease resistant trait in shrimp. Journal of Fisheries of China 26: 270–274. (in Chinese)

Nagaoka, T. and Y. Ogihara, 1997. Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theoretical and Applied Genetics 94: 597–602.

Nei, M., 1972. Genetic distance between populations. American Naturalist 106: 283–292.

Qian, W., S. Ge and D. Y. Hong, 2001. Genetic variation within and among populations of a wild rice Oryza granulata from China detected by RAPD and ISSR markers. Theoretical and Applied Genetics 102: 440–449.

Schaal, B. and L. Marlier, 1998. Maternal and paternal perception of individual odor signatures in human amniotic fluid—potential role in early bonding. Biology of the Neonate 74: 266–273.

Slatkin, M., 1987. Gene flow and the geographic structure of populations. Science 236: 787–792.

Tassanakajon, A., S. Pongsomboon, V. Rimphanitchayakit, P. Jarayabhand and V. Boonsaeng, 1997. Random amplified polymorphic DNA (RAPD) markers for determination of genetic variation in wild populations of the black tiger prawn (Penaeus monodon) in Thailand. Molecular Marine Biology and Biotechnology 6: 110–115.

Tsumura, Y., K. Ohba and S. H. Strauss, 1996. Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas-r (Pseudotsuga menziesii) and Sugi (Cryptomeria japonica). Theoretical and Applied Genetics 92: 40–45.

Uthicke, S. and S. Purcell, 2004. Preservation of genetic diversity in restocking of the sea cucumber Holothuria scabra investigated by allozyme electrophoresis. Canadian Journal of Fisheries and Aquatic Sciences 61: 519–528.

Wan, J. F., Z. M. Bao, G. X. Liu, J. Pan and R. C. Wang, 2004. Segregation of ISSR markers in hybrid larvae of two scallop species. High Technology Letters 15: 82–87. (in Chinese)

Wang, W. J., J. Kong, Z. M. Bao, J. Y. Deng and Z. M. Zhuang, 2001. Isozyme variation in four populations of Penaeus chinensis shrimp. Biodiversity Science 9: 241–246.

Wang, W. J. and J. Kong, 2002. Preliminary study on the application of inter-simple sequence repeats (ISSR)-PCR technique in Chinese shrimp (Fenneropenaeus chinensis). Marine Fisheries Research 23: 1–4. (in Chinese)

Yeh, F. C., R. C. Yang and T. Boyle, 1999. POPGENE. Microsoft Windows-based freeware for population genetic analysis. Release 1.31. University of Alberta, Edmonton.

Zhang, C. Y., Y. G. Wang, X. J. Rong, H. L. Sun and S. G. Dong, 2004. Natural resources, culture and problems of sea cucumber worldwide. Marine Fisheries Research 25: 89–97. (in Chinese)

Zietkiewicz, E., A. Rafalski and D. Labuda, 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176–183.

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