Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD

Feng You; Peijun Zhang; Keling Wang; Jianhai Xiang

doi:10.1007/s00343-007-0078-9

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Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD

Updated：2023-05-16

- Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD
- Journal of Oceanology and Limnology Vol. 25, Issue 1, Pages: 78-84(2007)
- Affiliations：
  
  Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-007-0078-9
  CLC：
- Published：2007
- Accepted：
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You, F., Zhang, P., Wang, K. et al. Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD., Chin. J. Ocean. Limnol. 25, 78–84 (2007). https://doi.org/10.1007/s00343-007-0078-9
DOI：

You, F., Zhang, P., Wang, K. et al. Genetic variation of natural and cultured stocks of Paralichthys olivaceus by allozyme and RAPD., Chin. J. Ocean. Limnol. 25, 78–84 (2007). https://doi.org/10.1007/s00343-007-0078-9 DOI：

摘要

Abstract

Population genetics of the left-eyed flounder

Paralichthys olivaceus

including natural and cultured stocks distributed in the coastal waters near Qingdao of eastern maritime China

was analyzed in allozyme and RAPD. The results showed that among total 29 gene loci of 15 isozymes

9 and 7 were polymorphic in natural and cultured stocks

respectively. The status of genetic diversity in P. olivaceus is low in terms of polymorphic loci in chi-square test and genetic departure index of Hardy-Weinberg equilibrium. More alleles in IDHP

CAT

GDH and Ldh-C allozymes were found in the fish

which could be used as markers in assortive breeding and distinguishing stock

population or species evolution. Total 88 and 86 RAPD bands ranging from 200 to 2 500 bp were recognized individually in average of 7.8–8.0 bands per primer. The genetic diversity in cultured stock is lower than that in natural ones showing an obviously decreasing genetic divergence. Therefore

effective countermeasures must be taken to protect genetic resources of marine cultured fishes. The 2 markers have their own pros and cons. Combining the 2 markers to investigate the genetic variation of populations is suggested. The results provide basic data of this flounder and they are useful for studying genetic improvement and genetic resources of the fish.

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references

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