Antioxidant and isozyme features of two strains of Laminaria japonica (Phaeophyceae)

You Wang; Xuexi Tang; Yongqi Li; Zhiming Yu

doi:10.1007/s00343-007-0067-z

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Antioxidant and isozyme features of two strains of Laminaria japonica (Phaeophyceae)

Updated：2023-05-16

- Antioxidant and isozyme features of two strains of Laminaria japonica (Phaeophyceae)
- Journal of Oceanology and Limnology Vol. 25, Issue 1, Pages: 67-72(2007)
- Affiliations：
  
  1. Marine Life College Ocean University of China
  2. Key Laboratory of Marine Ecology & Environmental Sciences Institute of Oceanography Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/s00343-007-0067-z
  CLC：
- Published：2007
- Accepted：
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Wang, Y., Tang, X., Li, Y. et al. Antioxidant and isozyme features of two strains of Laminaria japonica (Phaeophyceae)., Chin. J. Ocean. Limnol. 25, 67–72 (2007). https://doi.org/10.1007/s00343-007-0067-z
DOI：

Wang, Y., Tang, X., Li, Y. et al. Antioxidant and isozyme features of two strains of Laminaria japonica (Phaeophyceae)., Chin. J. Ocean. Limnol. 25, 67–72 (2007). https://doi.org/10.1007/s00343-007-0067-z DOI：

摘要

Abstract

Healthy sporophytes of two gametophyte mutants of Laminaria japonica with different heat resistances: kelp 901 (901

with comparatively stronger heat-resistance) and Rongcheng No.1 (RC

sensitive to heat stress)

were respectively collected during October to December 2002 from Yantai and Rongcheng Sea Farm in the Shandong Peninsula of China. The contents of some biochemical materials and antioxidant capacity were analyzed under controlled laboratory conditions to identify if there is any relation between the overall antioxidant capacity and the heat-resistance in L. japonica and to understand possible mechanism of heat-resistance. Results show that: (1) the overall antioxidant capacity in healthy sporophyte of 901

such as vitamin E

polyphenol

and ascorbic acid contents and the enzymatic activity of SOD

POD

CAT

Gpx

PPO

and PAL

were not always higher than that of RC under controlled laboratory conditions

and no significance (P>0.05) was shown in total antioxidant capacity (T-AOC) in 901 and RC. Result suggested that the difference in antioxidant capacity was not a decisive factor for different heat-resistances in L. japonica; (2) the simultaneous assay on isozymes was carried out using vertical polyacrylamide gel electrophoresis (PAGE). Considerable differences in peroxide (PRX)

malate dehydrogenase (MDH)

malic enzyme (ME)

polyphenol oxidase (PPO) and glutamate dehydrogenase (GDH) were obtained in 901 and RC from either the band number

relative mobility (R f )

or staining intensity

and ME could be used as an indicator to distinguish healthy sporophyte of 901 and RC under controlled laboratory conditions.

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