Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro

L. S. Dolmatova; M. G. Eliseykina; N. F. Timchenko; A. L. Kovaleva; O. A. Shitkova

doi:10.1007/BF02860423

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Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro

Updated：2023-05-16

- Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro
- Journal of Oceanology and Limnology Vol. 21, Issue 4, Pages: 293-304(2003)
- Affiliations：
  
  1. . I. Il'ichev Pacific Oceanological Institute,V
  2. Institute of Marine Biology
  3. Research Institute of Epidemiology and Microbiology
- Author bio：
- Funds：
- DOI：10.1007/BF02860423
  CLC：
- Online First：31 January 2013，
  
  Published：2003
- Accepted：
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Dolmatova, L.S., Eliseykina, M.G., Timchenko, N.F. et al. Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro., Chin. J. Ocean. Limnol. 21, 293–304 (2003). https://doi.org/10.1007/BF02860423
DOI：

Dolmatova, L.S., Eliseykina, M.G., Timchenko, N.F. et al. Generation of reactive oxygen species in different fractions of the coelomocytes of holothurianEupentacta fraudatrix in response to the thermostable toxin ofYersinia pseudotuberculosis in vitro., Chin. J. Ocean. Limnol. 21, 293–304 (2003). https://doi.org/10.1007/BF02860423 DOI：

摘要

Abstract

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea

Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml

but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP

TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml

respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment

and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST

lectin stimulated ROS to lesser extent than that with lectin alone. When catalase

TST

and lectin were added into the FPMC simultaneously

ROS increase was similar to that under lectin treatment alone. On the whole

data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes

and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians.

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references

Balfray, S. K., Maule, A. G., Iwama, G. K., 2001. Coho salmonOncorhynchus kisutch strain differences in disease resistance and non-specific immunity, following immersion challenges withVibrio anguillarium.Diseases of aquatic organisms 47: 39–48.

Beachamp, C. O., Fridovich, I., 1971. Superoxide dismutase: improved assays and an assay applicable to acrilamide gels.Anal. Biochem. 44: 276–287.

Bertheussen, K., Seljelid, R., 1978. Echinoid phagocytes in vitro.Experimental Cell Research 111: 401–412.

Campa-Cordova, A. I., Hernandez-Saavedra, N. Y., De Philippis, R. et al., 2002. Generation of superoxide anion and SOD activity in haemocytes and muscle of American white shrimp (Litopenaeus vannamei) as a response to β-glucan and sulphated polysaccharide.Fish and Shellfish Immunity 12: 353–366.

Chia, Fu-Sh., Xing, J., 1996. Echinoderm coelomocytes.Zoological Studies 35(4): 231–254.

Eliseykina, M. G., Bulgakov, A. A., Nazarenko, E. L. et al., 1999. Distribution of 32kDa mannan-specific lectin in tissue of the Far East holothurianStichopus japonica.Izvestiya Akademii Nauk Biological Series.2: 228–232. (in Russion)

Eliseykina, M. G., Magarlamov, Y., 2002. Morphology of coelomocytes of holothuriansApostichopus japonicus (Aspidochirota: Stichopodidae) andCucumaria japonica (Dendrochirota: Cucumariidae).Biologia morya 28(3): 214–219. (in Russion)

Engel, M., Bachmann, M., Schroder, H. C. et al., 1992. A novel galactose- and arabinose-specific lectin from the spongePellina semitubulosa: isolation, characterization and immunobiological properties.Biochimie 74(6): 527–537.

Gromykhina, N. Y., Kozlov, V. A., 1996. Non-prostaglandin-dependent mechanisms of synthesis and action of immuno-regulatory factors of macrophagal and non-macrophagal origin.Immunology 5: 29–34. (in Russion)

Hughes, T. K., Smith, E. M., Barnett, J. A. et al., 1991. LPS stimulated invertebrate hemocytes: a role for immunoreactive TNF and IL-1.Developmental and Comparative Immunology 15: 117–122.

Isaeva, V. V., Korenbaum, E. S., 1989. Defence functions of coelomocytes and immunity of echinoderms.Biologiya morya 6: 3–14. (in Russion)

Klimenko, N. A., Dygay, A. M., Bogdashin, I. V. et al., 1993. The effect of mast cells on interleukin-1 production by macrophages of exudates and bone narrow in inflammation.Bul. Exp. Biol. Med. 6: 599–600. (in Russion)

Logvinenko, A. A., Dolmatova, L. S., Timchenko, N. F. et al., 2001. Correction effect of peptide immunomodulator gangleen on the activity ofYersinia pseudotuberculosis thermoresistant toxin in oxidant and antioxidant human leukocyte system.Antibiotics and Chemotherapy 46(2): 17–21. (in Russion)

Melnikov, V. P., 1991. Test of nitro blue tetrazolium reduction in mononuclear phagocytes.Laboratornoe delo 8: 51–53. (in Russion)

Njamkepo, E., Pinot, F., Francois, D. et al., 2000. Adaptive responses of human monocytes infected byBordetella pertussis: the role of adenylate cyclase hemoysin.J. Cell. Physiology 183: 91–99.

Nogae, C., Makino, N., Hata, T. et al., 1995. Interleukin 1-alpha-induced expression of manganous superoxide dismutase reduces myocardial reperfusion injury in the rat.J. Mol. Cell. Cardiology 27(10): 2091–2099.

Odintsova, N. A., 2001. Bases of cultivation of marine invertebrate cells. Dalnauka, Vladivostok, p. 1–162.

Pipe, R. K., 1992. Generation of reactive oxygen metabolites by the haemocytes of the musselMytilus edulis.Developmental and Comparative Immunology 16(2/3): 111–122.

Roch, P., 1999. Defense mechanisms and disease prevention in farmed marine invertebrates.Aquaculture 172 (1–2): 125–145.

Timchenko, N. F., Dolmatova, L. S., Raznik, S. D. et al., 1998. Influence ofYersinia pseudotuberculosis thermostable toxin on the oxidizing and antioxidizing activity of human blood leukocytes.Zh. Microbiol. (Moscow)6: 16–20.

Tschischka, K., Abele, D., Portner, H. O., 2000. Mitochondrial oxyconformity and cold adaptation in the polychaetaNereis pelagica and the bivalveArctica islandica from the Baltic and White seas.J. Exp. Biol. 203: 3355–3368.

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