Isolation of plasmid from the blue-green algaSpirulina platensis

Qin Song; Tong Shun; Zhang Peijun; C. K. Tseng

doi:10.1007/BF02850862

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Isolation of plasmid from the blue-green algaSpirulina platensis

Updated：2023-05-16

- Isolation of plasmid from the blue-green algaSpirulina platensis
- Journal of Oceanology and Limnology Vol. 11, Issue 3, Pages: 285-288(1993)
- Affiliations：
  
  Experimental Marine Biology Laboratory Institute of Oceanology
- Author bio：
- Funds：
- DOI：10.1007/BF02850862
  CLC：
- Published：1993
- Accepted：
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Song, Q., Shun, T., Peijun, Z. et al. Isolation of plasmid from the blue-green algaSpirulina platensis, ., Chin. J. Ocean. Limnol. 11, 285–288 (1993). https://doi.org/10.1007/BF02850862
DOI：

Song, Q., Shun, T., Peijun, Z. et al. Isolation of plasmid from the blue-green algaSpirulina platensis, ., Chin. J. Ocean. Limnol. 11, 285–288 (1993). https://doi.org/10.1007/BF02850862 DOI：

摘要

Abstract

CCC plasmid was isolated from an economically important blue-green alga —Spirulina platensis (1.7×106 dalton from the S6 strain and 1.2×106 dalton from the F3 strain) using a rapid method based on ultrasonic disruption of algal cells and alkaline removal of chromosomal DNA. The difference in the molecular weight of the CCC DNAs from the two strains differing in form suggests that plasmid may be related with the differentiation of algal form. This modified method

which does not use any lysozyme

is a quick and effective method of plasmid isolation

especially for filamentous blue-green algae.

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Keywords

references

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Related Institution

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