

FOLLOWUS
Institute of Oceanology
Published:1991
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Fayi, L., Dehai, L. Nature of copper in parent prawn hepatopancreas., Chin. J. Ocean. Limnol. 9, 115–122 (1991). https://doi.org/10.1007/BF02850670
Copper content in the hepatopancreas of wild parentPanaeus orientalis was more than 500 μg/g (wet wt)
among which over 90% distributed in the cytosol. 99% of the Cu in the subcellular fraction distributed in a very low molecular weight (VLMW)pool.DE-11 anion exchange chromatography of the VLMW material indicated that about 83% of the Cu in the pool could not be absorbed in the exchanger. Some main compounds containing copper (accounting for 77% of the Cu in the cytosol) were isolated from the cytosol fraction using a CM-32 cation exchange column. Amino acid analysis of the compounds showed that histidine was a major component
accounting for 58.2% of the total amino acids in the compounds
and that the contents of lysine and serine were also higher (9.6% and 7.6%
respectively)
indicating that Cu in the material isolated by CM-32 perhaps mainly existed as a histidine-Cu complex
and that some of it could have complexed with serine
glysine and/or their peptides.
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