Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis

Gu Tian-qing; Zhang Hui-miao; Sun Shi-hua

doi:10.1007/BF02850536

Your Location：

Home >

Browse articles >

Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis

Updated：2023-05-16

- Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis
- Journal of Oceanology and Limnology Vol. 14, Issue 1, Pages: 27-30(1996)
- Affiliations：
  
  Institute of Botany
- Author bio：
- Funds：
- DOI：10.1007/BF02850536
  CLC：
- Published：1996
- Accepted：
Scan QR Code
Tian-qing, G., Hui-miao, Z. & Shi-hua, S. Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis, ., Chin. J. Ocean. Limnol. 14, 27–30 (1996). https://doi.org/10.1007/BF02850536
DOI：

Tian-qing, G., Hui-miao, Z. & Shi-hua, S. Isolation, purification and characterization of the hydrogen evolution promoting factor of hydrogenase ofSpirulina platensis, ., Chin. J. Ocean. Limnol. 14, 27–30 (1996). https://doi.org/10.1007/BF02850536 DOI：

摘要

Abstract

A component (s-factor) with obvious promoting effect on hydrogen evolution of hydrogenase has been isolated and extracted from a cell-free preparation ofSpirulina platensis. The effect of the s-factor in the reaction system is similar to that of Na2S2O4

but is coupled with light. The s-factor has the maximum absorption peak at 620 nm in the oxidized state

at 590 nm in the reduced state. The partially purified s-factor showed two bands by SDS-PAGE and is distinctly different from phycocyanin

which has no change of oxidized state and reduced state absorption spectra

and also has no promoting effect on hydrogenase ofSpirulina platensis under the light.

关键词

Keywords

references

Gu Tianqing, Wang Fazhu, 1984. Studies on hydrogen bySpirulina, platensis.Proc. Int. Seaweed Symp. 11: 467–470.

Gu Tianqing, Zhou Peizhen, 1987. Isolation and characterization of hydrogenase fromSpirulina platensis.Hydrobiologia 151/152: 557–561.

Herrero A, Floes E., Guerrero M. G., 1981. Regulation of nitrate reductase levels in the cyanobacteriaAnacystis nidulans, Anabaena sp. strain 7119, andNostoc sp strain 6719.J. Bacteria 145: 175–180.

Laemmli U. K., 1970. Cleavage of structural protein during the assembly of the head of bacteriophage T4.Nature 227: 686.

Maria J. L., Juan L. S., Krishana K. R., et al., 1979. Isolation and characterization of the hydrogenase activity from the non-heterocystous cyanobacteriumSpirulina maxima.Febs Lett. 98: 342–346.

Pinevitch V. V., Verzilin N. N., Mikhailiv A. A., 1970.Spirulina platensis-a new object of intensity mass cultivation.Fiziol. Rast. 17: 1037–1045. (in Russian). English translation inSov. Pi. Physiol.,17: 866–873.

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Cyanobacterial extracellular alkaline phosphatase: detection and ecological function

Influences of aeration induced turbulence on growth and competition of Microcystis and Scenedesmus in the presence of sediments with varying particle sizes

Observation and simulation of 2-methylisoborneol in the Qingcaosha Reservoir, Changjiang estuary

Polyphasic examination on Merismopedia tenuissima CHAB 7021 from Ganjiang River, China revealed the polyphyly of the genus Merismopedia (Cyanobacteria)

Molecular detection of microbial communities associated with Microcystis vs Synechococcus dominated waters in Tianjin, China

Related Author

Lingling WAN

Chunlei SONG

Yiyong ZHOU

Xiuyun CAO

Qiang HE

Yiyao WANG

Ming LI

Yizhong CHEN

Related Institution

Key Laboratory of Algal Biology, State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences

College of Natural Resources and Environment, Northwest A&F University, Yangling

Chengdu Environmental Protection Research Institute

Shanghai Academy of Environmental Sciences

State Key Laboratory of Estuarine and Coastal Research, East China Normal University

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰