The induction and extraction of metallothioneins inArtemia

Chen Bing-liang; Paul K. Chien

doi:10.1007/BF02850516

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The induction and extraction of metallothioneins inArtemia

Updated：2023-05-16

- The induction and extraction of metallothioneins inArtemia
- Journal of Oceanology and Limnology Vol. 12, Issue 2, Pages: 175-179(1994)
- Affiliations：
  
  University of San Francisco
- Author bio：
- Funds：
- DOI：10.1007/BF02850516
  CLC：
- Published：1994
- Accepted：
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Bing-liang, C., Chien, P.K. The induction and extraction of metallothioneins inArtemia, ., Chin. J. Ocean. Limnol. 12, 175–179 (1994). https://doi.org/10.1007/BF02850516
DOI：

Bing-liang, C., Chien, P.K. The induction and extraction of metallothioneins inArtemia, ., Chin. J. Ocean. Limnol. 12, 175–179 (1994). https://doi.org/10.1007/BF02850516 DOI：

摘要

Abstract

A small amount of heavy metal binding protein

identified by BioRad Protein Assay

has been isolated from the adult brine shrimp

Artemia franciscanus. This protein has an apparent molecular weight between 6000 to 9000 dalton. a UV absorption peak at 260 instead of 280 nm like most proteins; and has high affinity towards binding with radioactive labeled109Cd. These characteristics are similar to that of metallothioneins reported for many vertebrate and invertebrate

marine and terrestrial animals. After the brine shrimp is exposed to a small amount of Cd2+ for 24 h

a large amount of metallothionein can be isolated

showing the inducibility of this detoxifying protein in the adultArtemia in a short period of time.

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references

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