Structural studies on the oligomers from the polysaccharide ofGracilaria textorii (Rhodophyta) using β-agarase and13C-NMR spectroscopy

Ji Minghou; M. Lahaye; W. Yaphe

doi:10.1007/BF02850446

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Structural studies on the oligomers from the polysaccharide ofGracilaria textorii (Rhodophyta) using β-agarase and13C-NMR spectroscopy

Updated：2023-05-16

- Structural studies on the oligomers from the polysaccharide ofGracilaria textorii (Rhodophyta) using β-agarase and13C-NMR spectroscopy
- Journal of Oceanology and Limnology Vol. 8, Issue 2, Pages: 135-149(1990)
- Affiliations：
  
  1. Institute of Oceanology
  2. Department of Microbiology and Immunology McGill University
- Author bio：
- Funds：
- DOI：10.1007/BF02850446
  CLC：
- Published：1990
- Accepted：
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Minghou, J., Lahaye, M. & Yaphe, W. Structural studies on the oligomers from the polysaccharide ofGracilaria textorii (Rhodophyta) using β-agarase and13C-NMR spectroscopy., Chin. J. Ocean. Limnol. 8, 135–149 (1990). https://doi.org/10.1007/BF02850446
DOI：

Minghou, J., Lahaye, M. & Yaphe, W. Structural studies on the oligomers from the polysaccharide ofGracilaria textorii (Rhodophyta) using β-agarase and13C-NMR spectroscopy., Chin. J. Ocean. Limnol. 8, 135–149 (1990). https://doi.org/10.1007/BF02850446 DOI：

摘要

Abstract

The 60% ethanolic extract fromGracilaria textorii (Rhodophyta) was degraded with β-agarase

and certain charged (sulfated) and neutral oligosaccharides were separated by using DEAE Sephadex A25 and Bio- gel P6

P2 chromatographic techniques. Some of the charged oligomers were verified to be neoagarotetraose-63-sulfate (DP2)

neoagarohexaose-63

65-disulfate (DP3) and neoagarooctaose-63

67-trisulfate (DP4) by using13C- and1H-NMR spectroscopy. One neutral oligomer was assumed to be a mixture of methylated neoagarotetraoses (DP2) by1H-NMR spectroscopy. These oligomers assigned by their chemical shifts may be used as the model compounds for the structural investigation of the agar-type sulfated polysaccharides using the β-agarase degradation method.

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references

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