Renxue, W., Xianhan, W., Jing, Z. et al. The insemination of goldfish (Carassium auratus) oocyte maturedin vitro, ., Chin. J. Ocean. Limnol. 9, 88–93 (1991). https://doi.org/10.1007/BF02849794
DOI:
Renxue, W., Xianhan, W., Jing, Z. et al. The insemination of goldfish (Carassium auratus) oocyte maturedin vitro, ., Chin. J. Ocean. Limnol. 9, 88–93 (1991). https://doi.org/10.1007/BF02849794DOI:
The insemination of goldfish (Carassium auratus) oocyte maturedin vitro
摘要
Abstract
Full maturation of goldfish oocyte was inducedin vitro by 17 α-hydroxy-20β-dihydroprogesterone. The oocyte maturation involves GV migration to the periphery of the oocyte and germinal vesicle breakdown (GVBD). In the experiment
incubation duration for GVBD varied in different broods of oocytes. Generally
if the duration for GVBD was shorter than 6 h
oocytes would have a better chance to survive after maturation and insemination. The maturation of nucleus (GV) and cytoplasm are not synchronous. Cytoplasm maturation occurs several hs after GVBD. Oocytes inseminated 8–9 h after GVBD have the highest fertilizing and hatching rate. Fertilized ova maturedin vitro can develop to sexually mature adults capable of reproduction.
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references
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