Fatty acids profile in a high cell density culture of arachidonic acid-richParietochloris incisa (Trebouxiophyceae, chlorophyta) exposed to high PFD

Liu Jian-guo; Zvi Cohen; Amos Richmond

doi:10.1007/BF02849652

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Fatty acids profile in a high cell density culture of arachidonic acid-richParietochloris incisa (Trebouxiophyceae, chlorophyta) exposed to high PFD

Updated：2023-05-16

- Fatty acids profile in a high cell density culture of arachidonic acid-richParietochloris incisa (Trebouxiophyceae, chlorophyta) exposed to high PFD
- Journal of Oceanology and Limnology Vol. 20, Issue 2, Pages: 149-156(2002)
- Affiliations：
  
  1. Research and Development Center of Morine Biotechnology Institute of Oceanology Chinese Academy of Sciences
  2. Microalgal Biotechnology The Jacob Blaustein Institute for Desert Research
- Author bio：
- Funds：
- DOI：10.1007/BF02849652
  CLC：
- Published：2002
- Accepted：
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Jian-guo, L., Cohen, Z. & Richmond, A. Fatty acids profile in a high cell density culture of arachidonic acid-richParietochloris incisa (Trebouxiophyceae, chlorophyta) exposed to high PFD., Chin. J. Ocean. Limnol. 20, 149–156 (2002). https://doi.org/10.1007/BF02849652
DOI：

Jian-guo, L., Cohen, Z. & Richmond, A. Fatty acids profile in a high cell density culture of arachidonic acid-richParietochloris incisa (Trebouxiophyceae, chlorophyta) exposed to high PFD., Chin. J. Ocean. Limnol. 20, 149–156 (2002). https://doi.org/10.1007/BF02849652 DOI：

摘要

Abstract

The changes in arachidonic acid (AA) and fatty acids profiles along the growth curve ofParietochloris incisa

a coccoid snow green alga

were studied in a 2.8 cm light-path flat photobiorcactor

exposed to strong photon flux density [PFD

2400 μEmol/(m2·s)]. Sixteen fatty acids were identified by gas chromatography showing that AA was the dominant fatty acid (33%–41%) followed by linoleic acid (17%–21%). AA content was closely investigated with respect to total fatty acids (TFA)

ash free dry weight (AFDW) of cell mass as well as total culture content. These parameters were influenced significantly in a similar manner by culture growth phase

i.e.

slightly decreasing in the lag period

gradually increasing in the logarithmic phase

becoming maximal at the early stationary phase

starting to decrease at the late stationary phase

sharply dropping at the decline phase. The increase in AA per culture volume during the logarithmic phase was not only associated with the increase in AFDW but also connected with a corresponding increase in AA/TFA

TFA/AFDW as well as AA/AFDW. The sharp decrease in AA content of the culture during the decline phase was mainly due to the decrease in AA/TFA

TFA/AFDW and AA/AFDW

although AFDW declined only a small extent. Maximal AA concentration

obtained at the early stationary phase

was 900 mg/L culture volume

and the average daily net increase of AA during 9 days logarithmic growth was 1.7 g/(m2·day). Therefore

harvesting prior to the decline phase in a batch culture

or at steady state in continuous culture mode seems best for high AA production. The latter possibility was also further confirmed by continuous culture with 5 gradients of harvesting rate.

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references

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Related Author

Liu Jian-guo

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Related Institution

Research and Development Center of Marine Biotechnology Institute of Oceanology Chinese Academy of Sciences

Division of life Sciences Nanjing Normal University

Microalgal Biotechnology The Jacob Bloustein Institute for Desert Research Ben-Gurion University of Negev

Key Laboratory of Marine Biotechnology of Zhejiang Province, School of Marine Sciences, Ningbo University

Key Laboratory of Applied Marine Biotechnology, School of Marine Sciences, Ningbo University

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