Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor

Liu Jian-guo; David O. Hall; Krishna K. Rao; Anatoly A. Tsygankov; Dmitry A. Sveshnikov

doi:10.1007/BF02849089

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Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor

Updated：2023-05-16

- Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor
- Journal of Oceanology and Limnology Vol. 16, Issue 1, Pages: 118-126(1998)
- Affiliations：
  
  1. Institute. of Oceanology. Chinese Academy of Sciences
  2. Division of Life Sciences King’s College London
  3. Institute of Soil Sciences and Photosynthesis Russian Acad. of Sciences Pushchino
  4. Department of Plant Physiology Biological Faculty Moscow State University
- Author bio：
- Funds：
- DOI：10.1007/BF02849089
  CLC：
- Published：1998
- Accepted：
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Jian-guo, L., Hall, D.O., Rao, K.K. et al. Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor., Chin. J. Ocean. Limnol. 16, (Suppl 1), 118–126 (1998). https://doi.org/10.1007/BF02849089
DOI：

Jian-guo, L., Hall, D.O., Rao, K.K. et al. Hydrogen photoproduction ofA. Variabilis incorporated in a photobioreactor., Chin. J. Ocean. Limnol. 16, (Suppl 1), 118–126 (1998). https://doi.org/10.1007/BF02849089 DOI：

摘要

Abstract

H2 photoproduction and nitrogenase activities in two strains ofAnabaena variabilis marked wild type ATCC 29413 and mutant PK84 exposed to thermal stress (temperature higher than the normal incubation temperature of 30°C) were studied. Cultures of both strains collected from any interval of logarithmic growth phase exhibited high H2 photoproduction and nitrogenase activities when exposed to limited time heat shock during the assay process. In contrast

the algal H2 photoproduction rate of both strains fluctuated with long term thermal stress caused by increasing the growth temperature from 30°C to 36°C. The changes of nitrogenase (the key H2 photobiosynthetic enzyme) activities in the mutant PK84 showed variation tendency similar to that of H2 photoproduction during exposure to thermal stress

indicating that fluctuation of H2 photoproduction in the mutant was mainly due to the variation of nitrogenase activities. A temporary maximal H2 photoproduction in the mutant PK84 (wild type ATCC29413) was observed when cells grew at 36°C for 14 (6) days. However

the responses of nitrogenase activities in the wild type to thermal stress were not completely similar to those in the mutant in spite of similar variations of H2 photoproduction in both strains. The data obtained in these studies suggested that the activities of other enzymes (in the wild strain) involved in H2 photoproduction were affected by thermal stress since H2 photoproduction maximized or dropped to 0 without variation tendency similar to that of nitrogenase activities. Furthermore

an enhancement of H2 photoproduction speed of the mutant strain cultured in a 4.4 L laboratory photobioreactor was also observed when it was subjected to short time continuous charge of argon

and temperature rise. All these results indicated that high temperature plays an important role in the photo-autotrophic H2 photoproduction

and that long term thermal stress is unfavourable for net H2 photoproduction in both strains ofA. variabilis though short-time heat shock is conducive to H2 photoproduction.

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references

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Related Author

Liu Jian-guo

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Related Institution

Institute of Oceanology Chinese Academy of Sciences

Division of Life Sciences King's College London

Institute of Solid Science and Photosynthesis Russian Academy of Sciences Pushchino

Dept. of Plant Physiol. Biological Faculty Moscow State University

University of Chinese Academy of Sciences

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