Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant

Shin-ichi Yano; Yoshikazu Kawata; Hiroyuki Kojima

doi:10.1007/BF02849087

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Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant

Updated：2023-05-16

- Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant
- Journal of Oceanology and Limnology Vol. 16, Issue 1, Pages: 105-108(1998)
- Affiliations：
  
  Agency of Industrial Science and Technology Osaka National Research Institute Ikeda
- Author bio：
- Funds：
- DOI：10.1007/BF02849087
  CLC：
- Published：1998
- Accepted：
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Yano, Si., Kawata, Y. & Kojima, H. Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant., Chin. J. Ocean. Limnol. 16, (Suppl 1), 105–108 (1998). https://doi.org/10.1007/BF02849087
DOI：

Yano, Si., Kawata, Y. & Kojima, H. Extracellular release of β-lactamase by osmotic shock inSynechococcus transformant., Chin. J. Ocean. Limnol. 16, (Suppl 1), 105–108 (1998). https://doi.org/10.1007/BF02849087 DOI：

摘要

Abstract

A unicellular cyanobacteriumSynechococcus sp. strain PCC 7002 was transformed with plasmid pQL1

on which β-lactamase gene (bla) and β-galactosidase gene (lacZ) were encoded. The transformant cells released β-lactamase into medium by an abrupt drop of osmotic pressure. This result indicates that this cyanobacterium recognizes and processes the signal sequence of β-lactamase

and accumulates the enzyme in periplasm. Repeated release of β-lactamase was possible by repeated osmotic shocks wihout

impairing cell viability. On the other hand

most of the β-galactosidase remained in cytoplasm under the osmotic shock.

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references

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