Production of bioplastics and hydrogen gas by photosynthetic microorganisms

Yasuo Asada; Masato Miyake; Jun Miyake

doi:10.1007/BF02849086

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Production of bioplastics and hydrogen gas by photosynthetic microorganisms

Updated：2023-05-16

- Production of bioplastics and hydrogen gas by photosynthetic microorganisms
- Journal of Oceanology and Limnology Vol. 16, Issue 1, Pages: 91-104(1998)
- Affiliations：
  
  1. National Institute of Bioscience and Human-Technology AIST/MITI Higashi Tsukuba,shi
  2. National Institute for Advanced Interdisciplinary Research AIST/MITI Higashi Tsukuba,shi
- Author bio：
- Funds：
- DOI：10.1007/BF02849086
  CLC：
- Published：1998
- Accepted：
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Asada, Y., Miyake, M. & Miyake, J. Production of bioplastics and hydrogen gas by photosynthetic microorganisms., Chin. J. Ocean. Limnol. 16, (Suppl 1), 91–104 (1998). https://doi.org/10.1007/BF02849086
DOI：

Asada, Y., Miyake, M. & Miyake, J. Production of bioplastics and hydrogen gas by photosynthetic microorganisms., Chin. J. Ocean. Limnol. 16, (Suppl 1), 91–104 (1998). https://doi.org/10.1007/BF02849086 DOI：

摘要

Abstract

Our efforts have been aimed at the technological basis of photosynthetic-microbial production of materials and an energy carrier. We report here accumulation of poly-(3-hydroxybutyrate) (PHB)

a raw material of biodegradable plastics and for production of hydrogen gas

and a renewable energy carrier by photosynthetic microorganisms (tentatively defined as cyanobacteria plus photosynthetic bateria

in this report). A thermophilic cyanobacterium

Synechococcus sp. MA19 that accumulates PHB at more than 20% of cell dry wt under nitrogen-starved conditions was isolated and microbiologically identified. The mechanism of PHB accumulation was studied. A mesophilicSynechococcus PCC7942 was transformed with the genes encoding PHB-synthesizing enzymes fromAlcaligenes eutrophus. The transformant accumulated PHB under nitrogen-starved conditions. The optimal conditions for PHB accumulation by a photosynthetic bacterium grown on acetate were studied. Hydrogen production by photosynthetic microorganisms was studied. Cyanobacteria can produce hydrogen gas by nitrogenase or hydrogenase. Hydrogen production mediated by native hydrogenase in cyanobacteria was revealed to be in the dark anaerobic degradation of intracellular glycogen. A new system for light-dependent hydrogen production was targeted.In vitro andin vivo coupling of cyanobacterial ferredoxin with a heterologous hydrogenase was shown to produce hydrogen under light conditions. A trial for genetic trasformation ofSynechococcus PCC7942 with the hydrogenase gene fromClostridium pasteurianum is going on. The strong hydrogen producers among photosynthetic bacteria were isolated and characterized. Co-culture ofRhodobacter andClostriumdium was applied to produce hydrogen from glucose. Conversely in the case of cyanobacteria

genetic regulation of photosynthetic proteins was intended to improve conversion efficiency in hydrogen production by the photosynthetic bacterium

Rhodobacter sphaeroides RV. A mutant acquired by UV irradiation will be characterized for the mutation and for hydrogen productivity in comparison with the wild type strain. Some basic studies to develop photobioreactors are also introduced.

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references

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