High efficiency induction of callus and regeneration of sporophytes ofLaminaria japonica (Phaeophyta)

Wang Xi-hua; Qin Song; Li Xin-ping; Jiang Peng; Zeng Cheng-kui; Qin Mei

doi:10.1007/BF02849083

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High efficiency induction of callus and regeneration of sporophytes ofLaminaria japonica (Phaeophyta)

Updated：2023-05-16

- High efficiency induction of callus and regeneration of sporophytes ofLaminaria japonica (Phaeophyta)
- Journal of Oceanology and Limnology Vol. 16, Issue 1, Pages: 67-74(1998)
- Affiliations：
  
  1. Institute of Oceanology the Chinese Academy of Sciences
  2. Qufu Normal University
- Author bio：
- Funds：
- DOI：10.1007/BF02849083
  CLC：
- Published：1998
- Accepted：
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Xi-hua, W., Song, Q., Xin-ping, L. et al. High efficiency induction of callus and regeneration of sporophytes ofLaminaria japonica (Phaeophyta)., Chin. J. Ocean. Limnol. 16, (Suppl 1), 67–74 (1998). https://doi.org/10.1007/BF02849083
DOI：

Xi-hua, W., Song, Q., Xin-ping, L. et al. High efficiency induction of callus and regeneration of sporophytes ofLaminaria japonica (Phaeophyta)., Chin. J. Ocean. Limnol. 16, (Suppl 1), 67–74 (1998). https://doi.org/10.1007/BF02849083 DOI：

摘要

Abstract

Four media (PESI solid

MS liquid

MS solid and ASP-C-I solid medium) were used to induce callus from excised tissues of the kelpLaminaria japonica. Only PESI solid medium and MS solid medium produced calli. Modified MS solid medium supplemented with mannitol (3%

W/V)

yeast extract (0.1%

W/V)

VB2 (0.5 mg/ml)

VB12 (0.5 mg/ml)

kinetin (0.108 μg/ml) and NAA (1.860μg/ml) showed much better effect on callus induction than non-modified MS solid medium. After 24 days of induction 75.5% of tissues in PESI solid medium showed callus formation. For modified MS solid medium

after three months of induction 67.3% of tissues dedifferentiated into calli. No callus could be found after five months of induction in either MS liquid or ASP-C-I solid medium. When calli were squashed and cultured in N-P enriched autoclaved seawater

MS liquid medium and ASP12-NTA liquid medium (both modified with kelp extract)

differentiation of cells and regeneration of sporophytes were only observed in ASP12-NTA medium supplemented with kelp extract. Gametophyte-like filaments formed first

then eggs were released. It was suggested that sporophyte formation could be a process of parthenogenesis. Sterilization techniques in tissue culture ofL. japonica were also tested in this study.

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references

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