Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135

Yoshikazu Kawata; Shin-ichi Yano; Hiroyuki Kojima

doi:10.1007/BF02849075

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Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135

Updated：2023-05-16

- Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135
- Journal of Oceanology and Limnology Vol. 16, Issue 1, Pages: 17-24(1998)
- Affiliations：
  
  Agency of Industrial Science and Technology Osaka National Research Institute Ikeda
- Author bio：
- Funds：
- DOI：10.1007/BF02849075
  CLC：
- Published：1998
- Accepted：
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Kawata, Y., Yano, Si. & Kojima, H. Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135., Chin. J. Ocean. Limnol. 16, (Suppl 1), 17–24 (1998). https://doi.org/10.1007/BF02849075
DOI：

Kawata, Y., Yano, Si. & Kojima, H. Construction of a genomic DNA library with a TA vector and its application in cloning of the phytoene synthase gene from the cyanobacteriumSpirulina platensis M-135., Chin. J. Ocean. Limnol. 16, (Suppl 1), 17–24 (1998). https://doi.org/10.1007/BF02849075 DOI：

摘要

Abstract

An efficient and simple method for constructing a genomic DNA library using a TA cloning vector is presented. It is based on the sonicative cleavage of genomic DNA and modification of fragment ends withTaq DNA polymerase

followed by ligation using a TA vector. This method was applied for cloning of the phytoene synthase genecrt B fromSpirulina platensis. This method is useful when genomic DNA cannot be efficiently digested with restriction enzymes

a problem often encountered during the construction of a genomic DNA library of cyanobacteria.

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references

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