Molecular cloning and sequencing of a cDNA encoding partial putative molt-inhibiting hormone fromPenaeus chinensis

Wang Zai-zhao; Xiang Jian-hai

doi:10.1007/BF02848851

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Molecular cloning and sequencing of a cDNA encoding partial putative molt-inhibiting hormone fromPenaeus chinensis

Updated：2023-05-16

- Molecular cloning and sequencing of a cDNA encoding partial putative molt-inhibiting hormone fromPenaeus chinensis
- Journal of Oceanology and Limnology Vol. 20, Issue 3, Pages: 232-236(2002)
- Affiliations：
  
  Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02848851
  CLC：
- Published：2002
- Accepted：
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Zai-zhao, W., Jian-hai, X. Molecular cloning and sequencing of a cDNA encoding partial putative molt-inhibiting hormone fromPenaeus chinensis, ., Chin. J. Ocean. Limnol. 20, 232–236 (2002). https://doi.org/10.1007/BF02848851
DOI：

Zai-zhao, W., Jian-hai, X. Molecular cloning and sequencing of a cDNA encoding partial putative molt-inhibiting hormone fromPenaeus chinensis, ., Chin. J. Ocean. Limnol. 20, 232–236 (2002). https://doi.org/10.1007/BF02848851 DOI：

摘要

Abstract

Total RNA was extracted from eyestalks of shrimpPenaeus chinensis. Eyestalk cDNA was obtained from total RNA by reverse transcription. Reverse transcriptase-polymerase chain reaction (RT-PCR) was initiated using eyestalk cDNA and degenerate primers designed from the amino acid sequence of molt-inhibiting hormone from shrimpPenaeus japonicus. A specific cDNA was obtained and cloned into a T vector for sequencing. The cDNA consisted of 201 base pairs and encoding for a peptide of 67 amino acid residues. The peptide ofP. chinensis had the highest identity with molt-inhibiting hormones ofP. japonicus. The cDNA could be a partial gene of molt-inhibiting hormones fromP. chinensis. This paper reports for the first time cDNA encoding for neuropeptide ofP. chinensis.

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references

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