Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines

Guo Hua-rong; Zhang Shi-cui; Li Hong-yan; Tong Shang-liang; Xiang Jian-hai

doi:10.1007/BF02848850

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Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines

Updated：2023-05-16

- Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines
- Journal of Oceanology and Limnology Vol. 20, Issue 3, Pages: 226-231(2002)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Ocean University of Qingdao
- Author bio：
- Funds：
- DOI：10.1007/BF02848850
  CLC：
- Published：2002
- Accepted：
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Hua-rong, G., Shi-cui, Z., Hong-yan, L. et al. Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines., Chin. J. Ocean. Limnol. 20, 226–231 (2002). https://doi.org/10.1007/BF02848850
DOI：

Hua-rong, G., Shi-cui, Z., Hong-yan, L. et al. Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines., Chin. J. Ocean. Limnol. 20, 226–231 (2002). https://doi.org/10.1007/BF02848850 DOI：

摘要

Abstract

Three continuous marine fish cell lines of FG (i.e.

Flounder Gill) from flounder (Paralichthys olivaceus) gill

SPH (i.e.

Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e.

Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin

were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However

the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG

SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However

morphological changes were found in SPH and RSBF compared to their original tissues. Therefore

the cellular morphology was still plastic in the relatively stable culture conditions

and it was possible that change of LDH patterns was related to morphological changes of fish cellsin vitro.

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references

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