Triploidy induction in Pacific oysterCrassostrea gigas by caffeine with thermal shock

Zhang Guo-fan; Chang Ya-qing; Song Jian; Ding Jun; Shen Jin-kai; Wang Yong-ping

doi:10.1007/BF02848731

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Triploidy induction in Pacific oysterCrassostrea gigas by caffeine with thermal shock

Updated：2023-05-16

- Triploidy induction in Pacific oysterCrassostrea gigas by caffeine with thermal shock
- Journal of Oceanology and Limnology Vol. 16, Issue 3, Pages: 249-255(1998)
- Affiliations：
  
  Key Laboratory of Maricultural Ecology Ministry of Agriculture PRC
- Author bio：
- Funds：
- DOI：10.1007/BF02848731
  CLC：
- Published：1998
- Accepted：
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Guo-fan, Z., Ya-qing, C., Jian, S. et al. Triploidy induction in Pacific oysterCrassostrea gigas by caffeine with thermal shock., Chin. J. Ocean. Limnol. 16, 249–255 (1998). https://doi.org/10.1007/BF02848731
DOI：

Guo-fan, Z., Ya-qing, C., Jian, S. et al. Triploidy induction in Pacific oysterCrassostrea gigas by caffeine with thermal shock., Chin. J. Ocean. Limnol. 16, 249–255 (1998). https://doi.org/10.1007/BF02848731 DOI：

摘要

Abstract

Zygotes of Pacific oyster (Crassostrea gigas) were treated for triploid induction with caffeine (10 mmol/L

15mmol/L and 20mmol/L) in combination with thermal shocks (at 40 minute post-fertilization) lasting for 5 and 10 minutes. The highest yield of triploids

41.5%

was obtained from the treatment with 20 mmol/L caffeine at 34°C lasting 10 minutes. The triploid levels were less than 30.0% in other treatments. Triploid induction was more effective in treatment with 15–20 mmol/L caffeine at 34–38 °C than with lower concentrations of caffeine at temperatures below 32 °C. Our results suggest that triploid induction with caffeine in combination with thermal shocks is less efficient than some other methods reported previously.

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references

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