The in vitro effects of steroids, human chorionic gonadotropin and cyanoketone on germinal vesicle breakdown of striped mullet (Mugil cephalus L.) oocytes

Hong Wanshu; Peter Thomas

doi:10.1007/BF02848517

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The in vitro effects of steroids, human chorionic gonadotropin and cyanoketone on germinal vesicle breakdown of striped mullet (Mugil cephalus L.) oocytes

Updated：2023-05-16

- The in vitro effects of steroids, human chorionic gonadotropin and cyanoketone on germinal vesicle breakdown of striped mullet (Mugil cephalus L.) oocytes
- Journal of Oceanology and Limnology Vol. 5, Issue 1, Pages: 1-8(1987)
- Affiliations：
  
  1. Department of Oceanology Xiamen University
  2. Marine Science Institute
- Author bio：
- Funds：
- DOI：10.1007/BF02848517
  CLC：
- Published：1987
- Accepted：
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Wanshu, H., Thomas, P. The in vitro effects of steroids, human chorionic gonadotropin and cyanoketone on germinal vesicle breakdown of striped mullet (Mugil cephalus L.) oocytes., Chin. J. Ocean. Limnol. 5, 1–8 (1987). https://doi.org/10.1007/BF02848517
DOI：

Wanshu, H., Thomas, P. The in vitro effects of steroids, human chorionic gonadotropin and cyanoketone on germinal vesicle breakdown of striped mullet (Mugil cephalus L.) oocytes., Chin. J. Ocean. Limnol. 5, 1–8 (1987). https://doi.org/10.1007/BF02848517 DOI：

摘要

Abstract

The in vitro effects of steroids

human chorionic gonadotropin (HCG) and cyanoketone on germinal vesicle breakdown (GVBD) of striped mullet (Mugil cephalus L.) oocytes were investigated. All concentrations of HCG (5

50 I.U./ml)

progesterone and pregnenolone at the highest concentrations(lug/ml) were moderately effective in inducing GVBD

whereas 17β-estrodiol

cortisol

testosterone

11β-hydroxyandrostenedione and 11-ketotestosterone did not stimulate GVBD. 17α

20βdihydroxy-4-pregnen-3-one (17α

20βdiOHprog) and deoxycorticosterone (DOC) were the most potent steroids in stimulating final oocyte maturation. The results indicate that C21 hydroxylated steroids are potent inducers of final maturation in mullet. Further

co-incubations with 17β-estradiol

cortisol and testosterone did not alter the maturation-inducing effects of HCG or 17α

20βdiOHprog. Cyanoketone

a blocker of 3βHSD activity

was only partially effective in blocking GVBD induced by HCG. This suggests that Δ5 (pregnenolone derived) and Δ4 steroids may be involved in final oocyte maturation in this species.

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references

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