Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody

Zhang Yong-an; Nie Pin

doi:10.1007/BF02847923

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Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody

Updated：2023-05-16

- Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody
- Journal of Oceanology and Limnology Vol. 20, Issue 4, Pages: 332-337(2002)
- Affiliations：
  
  State Key Laboratory of Freshwater Ecology and Biotechnology and Laboratory of Fish Diseases Institute of Hydrobiology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02847923
  CLC：
- Published：2002
- Accepted：
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Yong-an, Z., Pin, N. Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody., Chin. J. Ocean. Limnol. 20, 332–337 (2002). https://doi.org/10.1007/BF02847923
DOI：

Yong-an, Z., Pin, N. Serum immunoglobulin of the mandarin fish,Siniperca chuatsi with development of polyclonal antibody., Chin. J. Ocean. Limnol. 20, 332–337 (2002). https://doi.org/10.1007/BF02847923 DOI：

摘要

Abstract

Serum immunoglobulin from the mandarin fish

or the so-called Chinese perch

Siniperca chuatsi (Basilewsky)

was successfully purified using affinity chromatography. Heavy and light chains were detected on electrophoresis gel

with molecular weights being estimated at 72 and 29 kDa

respectively. The tetrameric IgM ofS. chuatsi was calculated to be 808 kDa. The rabbit polyclonal antisera against the purifed immunoglobulin were developed and tested by Western blot analysis. The antisera reacted strongly with the heavy chains ofS. chuatsi immunoglobulin. Humoral immune responses of the mandarin fish can then be examined using the developed polyclonal antibody.

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references

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