Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF

Guo Hua-rong; Zhang Shi-cui

doi:10.1007/BF02847922

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Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF

Updated：2023-05-16

- Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF
- Journal of Oceanology and Limnology Vol. 20, Issue 4, Pages: 323-331(2002)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. College of Marine Life Sciences Ocean University of Qingdao
- Author bio：
- Funds：
- DOI：10.1007/BF02847922
  CLC：
- Published：2002
- Accepted：
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Hua-rong, G., Shi-cui, Z. Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF., Chin. J. Ocean. Limnol. 20, 323–331 (2002). https://doi.org/10.1007/BF02847922
DOI：

Hua-rong, G., Shi-cui, Z. Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF., Chin. J. Ocean. Limnol. 20, 323–331 (2002). https://doi.org/10.1007/BF02847922 DOI：

摘要

Abstract

A continuous marine fish cell line RSBF (i. c. Red Sea Bream Fin) was utilized to screen the cytotoxicity and genotoxicity of polyethylenimine (PEI) and nickel chloride (NiCl2) in this study on the deleterious effects of aquatic genotoxins on fish. At the 0.01 to 1 μg/ml concentration tested

PEI had acute toxicity to the treated RSBF cells (IC50=1.12

0.92

0.88 and 0.64 μg/ml PEI for time 0 h

24 h

48 h and 72 h after treatment

respectively) and markedly inhibited their proliferation in a dose-dependent manner. At the 0.001 to 5 μmol/L concentration tested

NiCl2 posed no acute toxicity but significantly stimulated their growth (107%–214% of control). Random amplified polymorphic DNA (RAPD) technique was used to detect the genotoxic effects of PEI and NiCl2 by comparing the RAPD banding patterns of the control and treated cells. RAPD analysis indicated that at the concentrations tested

PEI was more genotoxic than NiCl2 to RSBF cells; that there was a slight dose-dependent response in the genotoxic effect of PEI but not NiCl2; and that RAPD technique might provide a sensitive

non-specific genotoxic endpoint. And the potent cytotoxicity and genotoxicity of PEI on fish cells showed that we should be cautious in utilizing it as gene

vector in fish gene transfer and human gene therapy.

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references

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