Properties of proteolytic toxin ofVibrio anguilolarum from diseased flounder

Mo Zhao-lan; Chen Shi-yong; Zhang Pei-jun

doi:10.1007/BF02847921

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Properties of proteolytic toxin ofVibrio anguilolarum from diseased flounder

Updated：2023-05-16

- Properties of proteolytic toxin ofVibrio anguilolarum from diseased flounder
- Journal of Oceanology and Limnology Vol. 20, Issue 4, Pages: 316-322(2002)
- Affiliations：
  
  Experimental Biology Laboratory Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02847921
  CLC：
- Published：2002
- Accepted：
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Zhao-lan, M., Shi-yong, C. & Pei-jun, Z. Properties of proteolytic toxin ofVibrio anguilolarum from diseased flounder., Chin. J. Ocean. Limnol. 20, 316–322 (2002). https://doi.org/10.1007/BF02847921
DOI：

Zhao-lan, M., Shi-yong, C. & Pei-jun, Z. Properties of proteolytic toxin ofVibrio anguilolarum from diseased flounder., Chin. J. Ocean. Limnol. 20, 316–322 (2002). https://doi.org/10.1007/BF02847921 DOI：

摘要

Abstract

Extracellular products (ECP) produced byVibrio anguillarum strain M3 originally isolated from diseased flounder (Paralichthys olivaceus) were prepared. ECP of M3 showed gelatinase

casinase

amylase and haemolytic activity on agarose plates. High protease activity against azocasin was detected. Bacterium M2 showed highest growth and protease activity at 25°C. The protease present in ECP showed maximal activity at pH 8 and 55°C; was completely inactivated by application of 80°C heat for 30 min; was completely inhibited by EDTA and HgCl2

and was partially inhibited by PMSF

SDS

MnCl2 and iodoacetic acid; but not inhibited by CaCl2 and MgCl2. The ECP was toxic to flounder fish at LD50 values of 3.1 μg protein/g body weight. The addition of HgCl2 and application of heat at 50°C decreased the lethal toxicity of ECP. When heated at 100°C

ECP lethality to flounder was completely inhibited. After intramuscular injection of ECP into flounder

it showed evident histopathological changes including necrosis of muscle

extensive deposition of haemosiderin in the spleen

dilated blood vessels congested with numerious lymphocytes in the liver. These results showed that ECP protease was a lethal factor produced by the bacteriumV. anguillarum M3.

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references

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