Structural studies on agar fractions extracted sequentially from chinese red sea weeds:Gracilaria sjeostedtii, G. textorii andG. salicornia using13C-NMR and IR spectroscopy

Ji Minghou; M. Lahaye; W. Yaphe

doi:10.1007/BF02847829

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Structural studies on agar fractions extracted sequentially from chinese red sea weeds:Gracilaria sjeostedtii, G. textorii andG. salicornia using13C-NMR and IR spectroscopy

Updated：2023-05-16

- Structural studies on agar fractions extracted sequentially from chinese red sea weeds:Gracilaria sjeostedtii, G. textorii andG. salicornia using13C-NMR and IR spectroscopy
- Journal of Oceanology and Limnology Vol. 6, Issue 2, Pages: 87-103(1988)
- Affiliations：
  
  1. Institute of Oceanology
  2. Dept of Microbiology & Immunology McGill University
- Author bio：
- Funds：
- DOI：10.1007/BF02847829
  CLC：
- Published：1988
- Accepted：
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Minghou, J., Lahaye, M. & Yaphe, W. Structural studies on agar fractions extracted sequentially from chinese red sea weeds:Gracilaria sjeostedtii, G. textorii andG. salicornia using13C-NMR and IR spectroscopy., Chin. J. Ocean. Limnol. 6, 87–103 (1988). https://doi.org/10.1007/BF02847829
DOI：

Minghou, J., Lahaye, M. & Yaphe, W. Structural studies on agar fractions extracted sequentially from chinese red sea weeds:Gracilaria sjeostedtii, G. textorii andG. salicornia using13C-NMR and IR spectroscopy., Chin. J. Ocean. Limnol. 6, 87–103 (1988). https://doi.org/10.1007/BF02847829 DOI：

摘要

Abstract

The polysaccharides of three species of ChineseGracilaria (Rhodophyta) were extracted by sequential solvent extraction and the chemical structures of the fractions obtained were investigated by using13C-NMR and IR spectroscopy. The13C-NMR spectra showed that the main extract

cold water fraction

fromG. sjeostedtii consisted of repeating disaccharides of agarobiose and (1→3)-β-D-galactopyranosy 1-(1→4)-α-L-galactopyranose 6-sulfate

the latter being a precursor to agarobiose and converted to agarobiose after alkali modification. The component of the 60% ethanol fraction fromG. textorii was composed mainly of agarose 6-sulfate

6-O-methyl-agarose and some amount of agarose

whse structures remained unchanged after alkali treatment. The autoclave extract fromG. salicornia revealed a complex structure composed of 6-O-methyl-agarose

2-O-methyl-agarose

branched 4-O-methyl-L-galactose and agarose 4-sulfate. All the substituents in these agaroses were stable against alkali treatment. The alkali extracts fromG. sjeostedtii andG. textorii showed the distinct spectra of amylopectin-like floridean starch

while that fromG. salicornia showed a mixture of agaroses and floridean starch. From the IR spectra the relative contents of total sulfate

6-anhydro-galactose

4-sulfate and 6-sulfate in some fractions of polysaccharides of the algae were qualitatively estimated by the ratios of absorption bands of 1250/2920

930/2920

850/2920 and 820/2920

respectively.

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references

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