The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage

Zhang Yujiang; Zhan Songmei; Cao Pengli; Liu Ning; Chen Xuehong; Wang Yuejun; Wang Chunbo

doi:10.1007/BF02847161

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The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage

Updated：2023-05-16

- The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage
- Journal of Oceanology and Limnology Vol. 23, Issue 3, Pages: 357-362(2005)
- Affiliations：
  
  1. Medical College Qingdao University Qingdao
  2. Yellow Sea Fishery Research Institute Qingdao
- Author bio：
- Funds：
- DOI：10.1007/BF02847161
  CLC：
- Published：2005
- Accepted：
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Yujiang, Z., Songmei, Z., Pengli, C. et al. The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage., Chin. J. Ocean. Limnol. 23, 357–362 (2005). https://doi.org/10.1007/BF02847161
DOI：

Yujiang, Z., Songmei, Z., Pengli, C. et al. The polypeptide inChlamys farreri can protect human dermal fibroblasts from ultraviolet B damage., Chin. J. Ocean. Limnol. 23, 357–362 (2005). https://doi.org/10.1007/BF02847161 DOI：

摘要

Abstract

To investigate the effect of polypeptide fromChlamys farreri (PCF) on NHDFin vitro

we modeled oxidative damage on normal human dermal fibroblasts (NHDF) exposed to ultraviolet B (UVB). In this study

3-[4

5-Dimethylthiazol-2-yl]-2

5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) were tested to measure cell viability. Enzymes including superoxide dismutase (SOD)

glutathione peroxidase (GSH-PX)

catalase (CAT) and xanthine oxidase (XOD) were determined biochemically. Total antioxidative capacity (T-AOC) and anti-superoxide anion capacity (A-SAC) were also determined. Ultrastructure of fibroblasts was observed under transmission electron microscope. The results showed that: UVB (1.176×10−4 J/cm2) suppressed the growth of fibroblasts and the introduction of PCF (0.25%–1%) before UVB reduced the suppression in a concentration-dependent manner. PCF could enhance the activities of SOD

GSH-PX and T-AOC as well as A-SAC. Also PCF could inhibit XOD activity

while it did not affect CAT activity. Ultrastructure of fibroblasts were damaged after UVB irradiation

concentration-dependent PCF reduced the destructive effect of UVB on cells. These results indicated that PCF can protect human dermal fibroblasts from being harmed by UVB irradiation via its antioxidant proerty.

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references

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