Molecular identification ofProrocentrum (Dinophyceae) species

Zhang Cheng; Han Xiaotian; Zou Jingzhong; Yu Zhiming; Song Xiuxian; Christian Schuett

doi:10.1007/BF02847154

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Molecular identification ofProrocentrum (Dinophyceae) species

Updated：2023-05-16

- Molecular identification ofProrocentrum (Dinophyceae) species
- Journal of Oceanology and Limnology Vol. 23, Issue 3, Pages: 317-322(2005)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Biologische Anstalt Helgoland
- Author bio：
- Funds：
- DOI：10.1007/BF02847154
  CLC：
- Published：2005
- Accepted：
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Cheng, Z., Xiaotian, H., Jingzhong, Z. et al. Molecular identification ofProrocentrum (Dinophyceae) species., Chin. J. Ocean. Limnol. 23, 317–322 (2005). https://doi.org/10.1007/BF02847154
DOI：

Cheng, Z., Xiaotian, H., Jingzhong, Z. et al. Molecular identification ofProrocentrum (Dinophyceae) species., Chin. J. Ocean. Limnol. 23, 317–322 (2005). https://doi.org/10.1007/BF02847154 DOI：

摘要

Abstract

A fragment of a large sub-unit ribosomal DNA (LrDNA) of 12 strains ofProrocentrum species was amplified by polymerase chain reaction (PCR). The PCR products were digested by 3 restriction endonucleases (Cfo I

Hae III

and RSA I) and then resolved in agarose gels. Results show that different species had different RFLP patterns

except forP. arcuatum (ME 131)

which had the same pattern toP. micans (ME160 and 04). The same fragment of 19 strains of the genus was also amplified and subjected to denaturing gradient gel electrophoresis (DGGE). 11 different patterns were resolved. Different cultures of a same species had the same pattern. The results of RFLP and DGGE analyses showed that eight newly isolated epibenthicProrocentrum species were different from each other

and also from other cultured ones examined in this study.P arcuatum (ME132) could not be differentiated fromP. micans (ME160 and 04)

it was probably mis-identified

since they are quite different morphologically.P. redfieldii (ME138) could also not be distinguished formP. triestinium (ME132)

it should be regarded as a synonym ofP. triestinium. Unexpectedly

a restriction site was found inP. micans

compared with previous sequence data.

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references

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