Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis

Liu Hui-lian; Shuai Li; Duan De-lin; Xu Huai-shu

doi:10.1007/BF02846612

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Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis

Updated：2023-05-16

- Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis
- Journal of Oceanology and Limnology Vol. 20, Issue 1, Pages: 62-66(2002)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Department of Environmental Science Qingdao University
  3. Department of Marine Biology Ocean University of Qingdao
- Author bio：
- Funds：
- DOI：10.1007/BF02846612
  CLC：
- Published：2002
- Accepted：
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Hui-lian, L., Li, S., De-lin, D. et al. Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis, ., Chin. J. Ocean. Limnol. 20, 62–66 (2002). https://doi.org/10.1007/BF02846612
DOI：

Hui-lian, L., Li, S., De-lin, D. et al. Axenic culture of free-living conchocelis ofPorphyra yezoensis andPorphyra haitanensis, ., Chin. J. Ocean. Limnol. 20, 62–66 (2002). https://doi.org/10.1007/BF02846612 DOI：

摘要

Abstract

After discarding marine microorganisms from conchocelis ofPorphyra yezoensis andPorphyra haitanensis

their axenic cultures were obtained through treatment with antibiotics. Antibiotic disc tests were carried out to determine the effectiveness of each antibiotic in eliminating contaminating microorganisms. Five of 12 antibiotics tested were selected and used to produce the axenic cultures in this study

which showed that 200 μg/mL streptomycin

250 μg/mL penicillin

252 μg/mL kanamycin

30 μg/mL chloramphenicol were effective concentrations for eliminating microorganisms from conchocelis when antibiotics were added singly step by step; whereas simultaneous combination of 150 μg/mL streptomycin

250 (or 350) μg/mL penicillin

150 (or 250) μg/mL kanamycin

70 μg/mL neomycin and 200 μg/mL chloramphenicol was also effective for producing the axenic cultures. However

it seemed that the treatments with antibiotics applied individually were more feasible than those will all antibiotics added at the same time. This may be due to the combined inhibiting effect of antibiotics on the growth and development of conchocelis.

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references

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