Analysis of IL-2-like factor in lymphocyte culture supernatant of olive flounder,Paralichthys oliveaceus

Wu Riqin; Zhang Peijun; Li Jun; Xu Yongli

doi:10.1007/BF02845145

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Analysis of IL-2-like factor in lymphocyte culture supernatant of olive flounder,Paralichthys oliveaceus

Updated：2023-05-16

- Analysis of IL-2-like factor in lymphocyte culture supernatant of olive flounder,Paralichthys oliveaceus
- Journal of Oceanology and Limnology Vol. 23, Issue 1, Pages: 60-64(2005)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Institute of Postgraduate Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02845145
  CLC：
- Published：2005
- Accepted：
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Riqin, W., Peijun, Z., Jun, L. et al. Analysis of IL-2-like factor in lymphocyte culture supernatant of olive flounder,Paralichthys oliveaceus, ., Chin. J. Ocean. Limnol. 23, 60–64 (2005). https://doi.org/10.1007/BF02845145
DOI：

Riqin, W., Peijun, Z., Jun, L. et al. Analysis of IL-2-like factor in lymphocyte culture supernatant of olive flounder,Paralichthys oliveaceus, ., Chin. J. Ocean. Limnol. 23, 60–64 (2005). https://doi.org/10.1007/BF02845145 DOI：

摘要

Abstract

To study immune mechanism of fish lymphocyte we performed a proliferation assay and ELISA using monoclonal antibody against human IL-2. The result showed that an interleukin-2 (IL-2)-like factor was detected in the supernatant of plant haemoglutinin (PHA)-stimulated lymphocyte culture from peripheral blood

spleen and head kidney of olive flounder

Paralichthys olivaceus. The quantities of IL-2-like factor in the supernatant from different lymphoid tissues were quite different. The IL-2 like factor in the supernatant from cultured head kidney lymphocytes was much higher than those of peripheral blood lymphocytes and spleen lymphocytes (P<0.01). The IL-2 activity was found in either mouse thymocyte proliferation assay or flounder head kidney lymphocyte proliferation assay and shown to have obvious enhancing effect on proliferation of the above two types of cell. The recombinant human IL-2

(rhIL-2) was able to stimulate flounder thymocyte proliferation and used to detect the IL-2 receptor (IL-2R) on the surface of flounder lymphocyte. The cross-reaction between the lymphocytes of flounder peripheral blood and CD25(IL-2R) was detected with flow cytometry and shown that the percentage of CD25-positive cell in peripheral blood was 7.74±0.67%.

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references

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