Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio

M.S. Sawant; Zhang Shicui; Wang Qingyin

doi:10.1007/BF02845144

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Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio

Updated：2023-05-16

- Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio
- Journal of Oceanology and Limnology Vol. 23, Issue 1, Pages: 54-59(2005)
- Affiliations：
  
  1. College of Marine Life Sciences Ocean University of China
  2. Yellow Sea Fisheries Research Institute
- Author bio：
- Funds：
- DOI：10.1007/BF02845144
  CLC：
- Published：2005
- Accepted：
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Sawant, M., Shicui, Z. & Qingyin, W. Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio, ., Chin. J. Ocean. Limnol. 23, 54–59 (2005). https://doi.org/10.1007/BF02845144
DOI：

Sawant, M., Shicui, Z. & Qingyin, W. Effects of base analogues 5-bromouracil and 6-aminopurine on development of zebrafishDanio Rerio, ., Chin. J. Ocean. Limnol. 23, 54–59 (2005). https://doi.org/10.1007/BF02845144 DOI：

摘要

Abstract

Zebrafish (Danio rerio) genetic screens allow isolation of a wide array of problems in vertebrate biology. The effects of base analogues 5-bromouracil and 6-aminopurine on the development of zebrafish embryos are reported for the first time in this study. The early development of the zebrafish embryos was little affected by 5-bromouracil and 6-aminopurine

while the late development (organogenesis) was significantly impaired. Embryos exposed to 5-bromouracil mainly showed curled tail

wavy body

golden pigmentation and the mouth with protruding lower jaw. 6-aminopurine-treated embryos had defective anterior structures

curled tails and wavy body. RAPD analysis showed that the majority of 5-bromouracil- and 6-aminopurine-treated larvae and fish shared banding patterns in common with the control

suggesting that most mutagenesis induced by these agents are point mutations. However

some fish derived from 5-bromouracil-treated embryos had golden (gol) pigmentation; and RAPD analysis revealed that their band patterns differed from those of the control. Possibly

5-bromouracil can occasionally cause relatively extensive changes in the fish genome. The results of this study may provide valuable help for detailed studies of mutagenesis.

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references

Aebersold, P. M., 1976. Mutagenic mechanism of 5-bromodeoxyuridine in Chinese hamster cells.Mutat. Res. 36: 357–362.

Ando, H. and M. Mishina., 1998. Efficient mutagenesis of zebrafish by a DNA cross-linking agent.Neuroscience Letters 244: 81–84.

Chakrabarti, S., G. Streisinger, F. Singer, C. Walker, 1983. Frequency of γ-ray induced specific locus and recessive lethal mutations in male germ cells of the zebrafish,Brachydanio rerio.Genetics 103: 109–124.

Driever, W., L. Solnica-Krezel, A. F. Schier, S. C. F. Neuhauss, J. Malicki, D. L. Stemple, D. Y. R. Stainier, F. Zwartkruis, S. Abdelilah, Z. Rangini, J. Belak and C. Boggs, 1996. A genetic screen for mutations affecting embryogenesis in zebrafish.Development 123: 37–46.

Fadool, J. M., 2001. Understanding retinal cell fate determination through genetic manipulations.Progress in Brain Research 131: 541–554.

Grunwald, D. J., G. Streisinger, 1992. Induction of recessive lethal and specific locus mutations in the zebrafish with nitrosourca.Genet. Res. 59: 103–116.

Gont, L. K., H. Steinbeisser, B. Blumberg and E. M. De Robertis, 1993. Tail formation as a continuation of gastrulation: the multiple cell populations of the xenopus tailbud derive from the late blastopore lip.Development 119: 991–1004.

Hopkins R., L. Goodman and F. Myron, 1980. Deoxyribonucleotide pools, base pairing, and sequence configuration affecting bromodeoxyuridine-and 2-aminopurine- induced mutagenesis.Proc. Natl. Acad. Sci. USA. 77(4): 1801–1805.

Halpern, M. E., R. K. Ho, C. Walker and C. B Kimmel, 1993. Induction of muscle pioneers and floor plate is distinguished by the zebrafishno tail mutation.Cell 75: 99–111.

Hammerschmidt M., F. Pelegri, M. C. Mullins, D. A. Kane, M. Brand, F. J. M. van Eeden, M. Furutani-Seiki, M. Granato, P. Haffter, C. P. Heisenberg, Y. J. Jiang, R. N. Kelsh, J. Odenthal, R. M. Warga and C. Nusslein-Volhard, 1996. Mutations affecting morphogenesis during gastrulation and tail formation in the zebrafish,Danio rerio.Development 123: 143–151.

Haffter, P., J. Odenthal, M. C. Mullins, S. Lin, M. J. Farrell, E. Vogelsang, F. Haas, M. Brand, F. J. M. van Eeden, M. Furutani-Seiki, M. Granato, M. Hammerschmidt, C. P. Heisenberg, Y. J. Jiang, D. A. Kane, R. N. Kelsh, N. Hopkins and C. Nusslein-Volhard. 1996. Mutattions affecting pigmentation and shape of the adult zebrafish.Development, Genes and Evolution 206(4): 260–276.

Kaufman, E. R., R. L. Davidson, 1978. Bromodeoxyuridine mutagenesis in mammalian cells: Mutagenesis is independent of the amount of bromouracil in DNA. Proc.Natl. Acad. Sci. USA 75(10): 4982–4986.

Kaufman, E. R., R. L. Davidson, 1978b. Biological and biochemical effects of bromodeoxyuridine and deoxycytidine on Syrian hamster melanoma cells, Somat.Cell Genet. 4: 587–607.

Kaufman, E. R., 1987. Uncoupling of the induction of mutations and sister-chromatid exchanges by the replication of 5-bromouracil substituted DNA.Mut. Res. 176: 133–141.

Kimmel, C. B., D. A. Kane, C. Walker, R. M. Warga and M. B. Rothman, 1989. A mutation that changes cell movement and cell fate in the zebrafish embryo.Nature 337: 358–362.

Kelsh, R. N., M. Brand, Y. J. Jiang, C. P. Heisenberg, S. Lin, P. Haffter et al., 1996. Zebrafish pigmentation mutations and the processes of neural crest development.Development. 123: 369–389.

Lasken, R. S. and M. F. Goodman, 1984. The biochemical basis of 5-bromouracil induced mutagenesis.J. Biol. Chem. 259: 11491–11495.

Mullins, M. C., M. Hammerschmidt, P. Haffter and C. Nusslein-Volhard, 1994. Large scale mutagenesis in the zebrafish: in search of genes controlling development in a vertebrate.Current Biology 4: 189–202.

Merker, S. S., F. J. M. van. Eeden, M. E. Halpern, C. B. Kimmel and C. Nusslein-Volhard, 1994. No tail (ntl) is the zebrafish homologue of the mouse T (Brachyury) gene.Development 120: 1009–1015.

Moore, M. J., D. Leavitt, F. Alice, M. Shumate, P. Alatalo and J. J. Stegeman, 1994. A cell proliferation assay for small fish and aquatic invertebrates using bath exposure to bromodeoxyuridine.Aquatic Toxicology 30: 183–188.

Ronen, A., 1980. 2-Aminopurine.Mutation Res. 75: 1–47.

Riley, B. B., D. J. Grunwald, 1995. Efficient induction of point mutations allowing recovery of specific locus mutations in zebrafish.Proc. Natl. Acad. Scie. USA 92: 5997–6001.

Singer, B. and D. Grunberger, 1983. Molecular Biology of Mutagens and Carcinogens. Plenum Press, New York.

Streisinger, G., F. Singer, C. Walker, D. Knauber, N. Dower, 1986. Segregation analysis and gene-centromere distances in zebrafish.Genetics 112: 311–319.

Sowers, L. C., B. R. Shaw, M. L. Veigl and W. D. Sedwick, 1987. DNA base modification: Ionised base pairs and mutagenesis.Mutat. Res. 177: 201–218.

Snow, E. T. and M. Sankar, 1988. Role of carcinogen-modified deoxynucleotide precursors in mutagenesis.Mutat. Res. 200: 157–164.

Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual (Second Edition). Cold Spring Harbour Laboratory, New York: Cold Spring Harbor Laboratory Press.

Solnica, K. L., A. F. Schier, W. Driever, 1994. Efficient recovery of ENU-induced mutations from the zebrafish germline.Genetics 136: 1401–1420.

Sawant, M. S., S. Zhang, L. Li, 2001. Effect of salinity on development of zebrafish,Brachydanio rerio.Current Science 81(10): 1 347–1 350.

Tucker, A. S., J. M. W. Slack, 1995. The xenopus laevis tail-forming region.Development 121: 249–262.

Walker, C., G. Streisinger, 1983. Induction of mutations by γ-rays in pregonial germ cells of zebrafish embryos.Genetics 103: 125–136.

Westerfield, M., 1994. The Zebrafish Book. University of Oregon Press, Eugene, Oregon.

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