Isolation and cultivation of the vegetative cells ofPorphyra haitanensis

Wang Sujuan; Wang Guangyuan; Sun Yunlong; Lu Anming

doi:10.1007/BF02843815

Your Location：

Home >

Browse articles >

Isolation and cultivation of the vegetative cells ofPorphyra haitanensis

Updated：2023-05-16

- Isolation and cultivation of the vegetative cells ofPorphyra haitanensis
- Journal of Oceanology and Limnology Vol. 5, Issue 4, Pages: 333-339(1987)
- Affiliations：
  
  Shanghai Fisheries University
- Author bio：
- Funds：
- DOI：10.1007/BF02843815
  CLC：
- Published：1987
- Accepted：
Scan QR Code
Sujuan, W., Guangyuan, W., Yunlong, S. et al. Isolation and cultivation of the vegetative cells ofPorphyra haitanensis, ., Chin. J. Ocean. Limnol. 5, 333–339 (1987). https://doi.org/10.1007/BF02843815
DOI：

Sujuan, W., Guangyuan, W., Yunlong, S. et al. Isolation and cultivation of the vegetative cells ofPorphyra haitanensis, ., Chin. J. Ocean. Limnol. 5, 333–339 (1987). https://doi.org/10.1007/BF02843815 DOI：

摘要

Abstract

This research deals mainly with the use of the vegetative cells of a marine red algaPorphyra haitanensis (laver) as seeds and their culture into young thalli for further cultivation in the sea. The experimental process was as follows. Enzymatically isolated vegetative cells were attached to rope substrates and cultured in the laboratory for a month until they became about 0.2–0.5 cm long sporelings and were then attached (7–15 sporelings per cm of rope) to nets for removal to the open sea for cultivation. A month after culturing

the thalli reached a maximum length of 50 cm (average of 20–30 cm). The thalli grew faster as the water temperature dropped from 21°C to 17°C. It was proved that the vegetative cells isolated from a small thallus about 5 cm long could grow up into normal thalli after being kept frozen for a year. The results of this study show that vegetative cells can be used as new seeds to simplify the production of laver sporelings. This technique is a significant advance in the field ofPorphyra culture.

关键词

Keywords

references

Fujian Province Bureau of Fisheries, 1979. Artificial Culture ofPorphyra haitanensis. People’s Press of Fujian Province, pp. 28–33, 65–78. (in Chinese)

Fujita, Y. and Migita, S., 1985. Isolation and culture of protoplasts from some seaweeds.Bull. Fac. Fish. Nagasaki Univ. 57: 39–45.4

Institute of Oceanology, Academia Sinica, 1978. Artificial Culture ofPorphyra yezoensis. Science and Technology Press, Beijing, pp. 59–61, 88–93. (in Chinese)

M. Polne-Fuller, M. Biniaminov and A. Gibor, 1984. Vegetative propagation ofPorpnyra perforata.Hydrobiologia 116/117:308–313.

Saga, N., 1984. Isolation of protoplasts from edible seaweeds.Bot. Mag. Tokyo 97: 423–427.

Saga, N., and Sakai, Y., 1984. Isolation of protoplasts fromLaminaria andPorphyra.Bull. Jap. Soc. Sci. Fish. 50: 1085.

Tang Yanlin, 1982. Isolation and cultivation of the vegetative cells and protoplasts ofPorphyra suborbiculata Kjellm.Journal of Shandong College of Oceanology 6(4): 45–50. (in Chinese)

Wang Sujuan, Zhang Xiaoping, Xu Zhidong and Sun Yunlong, 1986. Study on the cultivation of cells and protoplasts ofPorphyra haitanensis.Oceanologia et Limnologia Sinica 17(3):217–221. (in Chinese with English abstract)

Wang Sujuan, Xu Zhidong, Wang Guangyuan and Xia Zhenao, 1986. Ultrastructural study on protoplasts ofPorphyra haitanensis.Marine Sciences 10(4): 21–24. (in Chinese with English abstract)

Zhao Huandeng and Zhang Xuecheng, 1984. On the cultivation of the isolated vegetative cells ofPorphyra yezoensis Ueda.Journal of Fisheries of China 8(3): 197–202. (in Chinese)

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

On two new species ofGardnerula (Cyanophyta) and the taxonomy of the genus

Early stage differentiation of thallus cells ofPorphyra haitanensis (Rhodophyta)

Experimental Study of TBCC Engine Performance inLow Speed Wind Tunnel

Crack Growth Rate Model Based on Modal Frequencyfor a V-Notched Beam

Effects of Rake Measurements on Turbine Efficiency

Related Author

C. K. Tseng

Hua Maosen

Wang Guangyuan

Related Institution

Institute of Oceanology Academia Sinica

Shanghai Institute of Plant Physiology

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰