Preparation of polysaccharides in different molecular weights fromUlva pertusa Kjellm (Chorophyta)

Yu Pengzhan; Zhang Quanbin; Zhang Hong; Niu Xizhen; Li Zhien

doi:10.1007/BF02843632

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Preparation of polysaccharides in different molecular weights fromUlva pertusa Kjellm (Chorophyta)

Updated：2023-05-16

- Preparation of polysaccharides in different molecular weights fromUlva pertusa Kjellm (Chorophyta)
- Journal of Oceanology and Limnology Vol. 22, Issue 4, Pages: 381-385(2004)
- Affiliations：
  
  Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02843632
  CLC：
- Published：2004
- Accepted：
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Pengzhan, Y., Quanbin, Z., Hong, Z. et al. Preparation of polysaccharides in different molecular weights fromUlva pertusa Kjellm (Chorophyta)., Chin. J. Ocean. Limnol. 22, 381–385 (2004). https://doi.org/10.1007/BF02843632
DOI：

Pengzhan, Y., Quanbin, Z., Hong, Z. et al. Preparation of polysaccharides in different molecular weights fromUlva pertusa Kjellm (Chorophyta)., Chin. J. Ocean. Limnol. 22, 381–385 (2004). https://doi.org/10.1007/BF02843632 DOI：

摘要

Abstract

As molecular weight controls the biological activities of polysaccharides

screening the optimal molecular weight of polysaccharides is important in drug research and application. In this study

hydrogen peroxide was employed as oxidant

and temperature

reaction time

and concentration of polysaccharides and hydrogen peroxide were examined for their effects on the preparation of polysaccharides in different molecular weights fromUlva pertusa. Our experiment suggested that the optimal degradation concentrations for polysaccharides and hydrogen peroxide were 2.5% (w/v) and 8.0% (v/v)

respectively. The range of degradation measured by relative viscosity was mainly controlled by temperature and time. Results revealed that 35°C was the optimal temperature for obtaining low-degradation samples

and 50°C was the most favorable temperature to accelerate the reaction to yield highly-degraded samples. Four samples in different molecular weights A

C and D were finally prepared. The controllability was evaluated by the relative standard deviation (RSD) of relative viscosity

and the peak molecular weights and the polydispersity indexes (Mw/Mn) of molecular weights were measured by high performance gel permeation chromatography (HPGPC).

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references

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