Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium

Mei Junxue; Shen Songdong; Jiang Ming; Fei Xiugeng

doi:10.1007/BF02843147

Your Location：

Home >

Browse articles >

Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium

Updated：2023-05-16

- Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium
- Journal of Oceanology and Limnology Vol. 21, Issue 2, Pages: 166-170(2003)
- Affiliations：
  
  1. Marine Biotechnology Department Shandong University Weihai Branch
  2. Biology Department Suzhou University West Campus
  3. Qingdao Ocean University
  4. Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02843147
  CLC：
- Published：2003
- Accepted：
Scan QR Code
Junxue, M., Songdong, S., Ming, J. et al. Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium., Chin. J. Ocean. Limnol. 21, 166–170 (2003). https://doi.org/10.1007/BF02843147
DOI：

Junxue, M., Songdong, S., Ming, J. et al. Ultrastructure of single cells, callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium., Chin. J. Ocean. Limnol. 21, 166–170 (2003). https://doi.org/10.1007/BF02843147 DOI：

摘要

Abstract

It had been demonstrated that individual cells or protoplasts isolated fromPorphyra thallus by enzyme could develop into normal leafy thalli in the same way as monospores

and that isolated cells develop in different way in liquid and on semi-solid media. The authors observed the ultrastructure of isolated vegetative cells cultured on semi-solid media and compared them with those of monospores and isolated cells cultured in liquid media. The results showed that subcellular structures were quite different among cells in different conditions. In their development

isolated cells on semi-solid media did not show the characteristic subcellular feature of monospore formation

such as production of fibrous vesicles. Callus-like cells formed on semi-solid media underwent a distinctive modification in cellular organization. They developed characteristic cell inclusions and a special 2-layer cell covering. Golgi bodies

starch grains

mitochondria. Vacuoles were not commonly found in them.

关键词

Keywords

references

Chen, L. C. M., 1989. Cell suspension culture fromPorphyra linearis (Rhodophyta), a multicellular marine red alga.J. Appl. Phycol. 1: 153–159.

Chen, L. C. M., 1997.Porphyra cell suspension cultures, novel biotechnological approach for the production of hoshi-nori. Nat. Hist. Res., Natural History Museum and Institute, Chiba, Japan. Special Issue No.C3: 111–121.

Gall, E. A., Chiang, Y. M., Kloareg, B., 1993. Isolation and regeneration of protoplasts fromPorphyra dentata-and, Porphyra crispata.Eur. J. Phycol. 28: 277–283.

Hawkes, M. W., 1980. Ultrastructure characteristics of monospore formation inPorphyra gardneri (Rhodophyta).J. Phycol. 16: 192–196.

Mei, J. X. and Fei, X. G., 2001. The ultrastructure of separated and cultured cell ofPorphyra yezoensis.Chin. J. Oceanol. Limnol. 19(1): 35–39.

Polne-Fuller, M., Biniamonov, M., Gibor, A., 1984. Vegetative propagation ofPorphyra perforata.Hydrobiologia. 116/117: 308–313.

Pueschel, C. M., 1990. Cell culture.In: Cole K. M., Sheath R. G. eds., Biology of the Red Algae. Cambridge University Press, New York, p. 8–36.

Wang, S. J., Yan, X. H., 1990. Observation on the monospore-like cells in the somatic cell culture ofPorphyra haitanensis.Oceanologia et Limnologia Sinica. 21(3): 166–169. (in Chinese with English Abstract)

Zhao, H., Zhang, X., 1981. Isolation and cultivation of the vegetative cells ofPorphyra yezoensis Ueda.J. Shandong College Oceanogr. 11(1): 61–66. (in Chinese)

Views

Downloads

CSCD

Alert me when the article has been cited

Submit

Tools

Publicity Resources

Molecular phylogeny and ultrastructure of a new population of Urostyla grandis (Ciliophora, Hypotrichida) from Harbin, China

High efficiency of protoplast preparation for artificially cultured Ulva prolifera (Ulvophyceae, Chlorophyta)

Studies on color type variants from mutagenized protoplasts ofPorphyra haitanensis Chang et Zheng &P. Yezoensis ueda (rhodophycease)

Transient expression of exogenous gus gene inPorphyra yezoensis (Rhodophyta)

Effect of cytokinins and auxins on the growth of free-living conchocelis ofPorphyra yezoensis

Related Author

Zijian QIU

Xuming PAN

Lijie YU

Saleh A. AL-FARRAJ

Jing GAO

Xiaolu ZHAO

Ying CHEN

Xinglong CAI

Related Institution

Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia

Institute of Evolution & Marine Biodiversity, Ocean University of China

Laboratory of Protozoology, Harbin Normal University

University of Chinese Academy of Sciences

Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology

Address：Editorial Office of JOL, 88 Haijun Rd. Qingdao, 266000, China
Tel：+86-532-82898754 Email：jol@qdio.ac.cn
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备09064830号-19 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰