Assessing the germplasm ofLaminaria (phaeophyceae) with random amplified polymorphic DNA (RAPD) method

He Yingjun; Zou Yuping; Wang Xiaodong; Zheng Zhiguo; Zhang Daming; Duan Delin

doi:10.1007/BF02843144

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Assessing the germplasm ofLaminaria (phaeophyceae) with random amplified polymorphic DNA (RAPD) method

Updated：2023-05-16

- Assessing the germplasm ofLaminaria (phaeophyceae) with random amplified polymorphic DNA (RAPD) method
- Journal of Oceanology and Limnology Vol. 21, Issue 2, Pages: 141-148(2003)
- Affiliations：
  
  1. Institute of Oceanology Chinese Academy of Sciences
  2. Institute of Botany Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02843144
  CLC：
- Published：2003
- Accepted：
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Yingjun, H., Yuping, Z., Xiaodong, W. et al. Assessing the germplasm ofLaminaria (phaeophyceae) with random amplified polymorphic DNA (RAPD) method., Chin. J. Ocean. Limnol. 21, 141–148 (2003). https://doi.org/10.1007/BF02843144
DOI：

Yingjun, H., Yuping, Z., Xiaodong, W. et al. Assessing the germplasm ofLaminaria (phaeophyceae) with random amplified polymorphic DNA (RAPD) method., Chin. J. Ocean. Limnol. 21, 141–148 (2003). https://doi.org/10.1007/BF02843144 DOI：

摘要

Abstract

Eighteen gametophytes includingL. japonica

L. ochotensis andL. longissima

were verified with random amplified polymorphic DNA (RAPD) technique. Eighteen ten-base primers were chosen from 100 primers selected for final amplification test. Among the total of 205 bands amplified

181 (88.3%) were polymorphic. The genetic distance among different strains ranged from 0.072 to 0.391. The dendrogram constructed by unweighted pair-group method with arithmetic (UPGMA) method showed that the female and male gametophytes of the same cell lines could be grouped in pairs respectively. It indicated that RAPD analysis could be used not only to distinguish different strains ofLaminaria

but also to distinguish male and female gametophyte within the same cell lines. There is ambiguous systematic relationship if judged merely by the present data. It seems that the use of RAPD marker is limited to elucidation of the phylogenetic relationship among the species ofLaminaria.

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references

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