Purification and characterization of lectin from humoral fluids ofCharybdis feriatus

Dai Congjie; Wang Guizhong; He Jianfeng; Li Shaojing; Huang Huiyang

doi:10.1007/BF02842855

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Purification and characterization of lectin from humoral fluids ofCharybdis feriatus

Updated：2023-05-16

- Purification and characterization of lectin from humoral fluids ofCharybdis feriatus
- Journal of Oceanology and Limnology Vol. 24, Issue 4, Pages: 390-394(2006)
- Affiliations：
  
  1. Department of Oceanography State key Lab of Marine Environmental Sciences Xiamen University
  2. Polar Research Institute of China
  3. Department of Biology Quanzhou Normal College
- Author bio：
- Funds：
- DOI：10.1007/BF02842855
  CLC：
- Online First：31 January 2013，
  
  Published：2006
- Accepted：
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Congjie, D., Guizhong, W., Jianfeng, H. et al. Purification and characterization of lectin from humoral fluids ofCharybdis feriatus, ., Chin. J. Ocean. Limnol. 24, 390–394 (2006). https://doi.org/10.1007/BF02842855
DOI：

Congjie, D., Guizhong, W., Jianfeng, H. et al. Purification and characterization of lectin from humoral fluids ofCharybdis feriatus, ., Chin. J. Ocean. Limnol. 24, 390–394 (2006). https://doi.org/10.1007/BF02842855 DOI：

摘要

Abstract

To search new sources of lectin

an experiment on lectin distribution in humoral serum of crabCharybdis feriatus (in short

CFL) was conducted March

2002. When adding solid ammonium sulfate into the fluids up to 50% saturation at 4°C

most CFL activity showed precipitates who were then continually extracted by ammonium sulfate of different concentrations. The supernatant

which was called primary CFL fluids

was given a 17.60-fold purification and 45.70% recovery of total activity. Finally

by using Sephadex G-100 column chromatography

the CFL in the primary CFL fluid was highly purified. Compared to the original humoral fluids

the last purified CFL got a 203.90-fold purification and 30.48% recovery of total activity

and demonstrated a single band on SDS-PAGE. In the same time

the purified CFL was detected for agglutination activity with 7 kinds of animal erythrocytes. Other characterization

such as sugar inhibition

and the effect of temperature on the agglutination activity of CFL were also studied. Our results indicate that agglutination activity of CFL was influenced by sugar and temperature.

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Keywords

references

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