Tetraploid induction by inhibiting mitosis I in scallopChlamys farreri

Yang Hui-ping; Guo Xi-ming; Chen Zai-zhong; Wang Yong-ping

doi:10.1007/BF02842829

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Tetraploid induction by inhibiting mitosis I in scallopChlamys farreri

Updated：2023-05-16

- Tetraploid induction by inhibiting mitosis I in scallopChlamys farreri
- Journal of Oceanology and Limnology Vol. 17, Issue 4, Pages: 350-358(1999)
- Affiliations：
  
  1. Experiment Marine Biology Laboratory Institute of Oceanology Chinese Academy of Sciences
  2. Haskin Shellfish Research Laboratory Rutgers University
  3. Fisheries College Ocean University of Qingdao
- Author bio：
- Funds：
- DOI：10.1007/BF02842829
  CLC：
- Published：1999
- Accepted：
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Hui-ping, Y., Xi-ming, G., Zai-zhong, C. et al. Tetraploid induction by inhibiting mitosis I in scallopChlamys farreri, ., Chin. J. Ocean. Limnol. 17, 350–358 (1999). https://doi.org/10.1007/BF02842829
DOI：

Hui-ping, Y., Xi-ming, G., Zai-zhong, C. et al. Tetraploid induction by inhibiting mitosis I in scallopChlamys farreri, ., Chin. J. Ocean. Limnol. 17, 350–358 (1999). https://doi.org/10.1007/BF02842829 DOI：

摘要

Abstract

Tetraploid induction was carried out by inhibiting mitosis I in fertilized eggs ofChlamys farreri. Mitosis I was blocked with cold shock (5–7°C)

Cytochalasin B (0.75 mg/L) and 6-dimethylaminopurine (6-DMAP) (60–75 mg/L) when 60% fertilized eggs released polar body II at 20°C. At 4-cells embryo stage

the ploidy was determined by counting chromosome number. In control groups

most embryos were diploids (72.22%) and aneuploids (24.78%). In Cytochalasin B

cold shock and 6-DMAP treated groups

tetraploids were respectively 10.51%

4.08%

and 13.34%; aneuploids were 43.10%

35.93% and 29.16%

and triploids were 7.84%

8.52% and 18.33%. At D-larva stage

ploidy was determined by flow cytometry (FCM). The ploidy analysis of day 2 larvae showed diploids in control group and also in three treated groups. Juvenile scallops (0.2–0.3cm) which were harvested in two control groups and two CB treated groups were all diploids through checking ploidy individually by FCM.

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references

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