A fast and indirect fluorescent antibody assay for the vibrio in large yellow croakerPseudosciaena crocea (Richardson)

Wang Jun; Su Yongquan; Yan Qingpi

doi:10.1007/BF02842766

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A fast and indirect fluorescent antibody assay for the vibrio in large yellow croakerPseudosciaena crocea (Richardson)

Updated：2023-05-16

- A fast and indirect fluorescent antibody assay for the vibrio in large yellow croakerPseudosciaena crocea (Richardson)
- Journal of Oceanology and Limnology Vol. 21, Issue 1, Pages: 91-96(2003)
- Affiliations：
  
  1. Dept. of Oceanography & Institute of Subtropical Ocean Xiamen University
  2. Institute of Aquaculture Biotechnology & Fishery College Jimei university
- Author bio：
- Funds：
- DOI：10.1007/BF02842766
  CLC：
- Published：2003
- Accepted：
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Jun, W., Yongquan, S. & Qingpi, Y. A fast and indirect fluorescent antibody assay for the vibrio in large yellow croakerPseudosciaena crocea (Richardson)., Chin. J. Ocean. Limnol. 21, 91–96 (2003). https://doi.org/10.1007/BF02842766
DOI：

Jun, W., Yongquan, S. & Qingpi, Y. A fast and indirect fluorescent antibody assay for the vibrio in large yellow croakerPseudosciaena crocea (Richardson)., Chin. J. Ocean. Limnol. 21, 91–96 (2003). https://doi.org/10.1007/BF02842766 DOI：

摘要

Abstract

A fast and indirect fluorescent antibody assay for theVibrio alginolyticus andV. parahaemolyticus infecting the large yellow croaker has been developed. The specific antisera for the two strains of vibrio were prepared with New Zealand rabbit and the antiserum and cross-reactive efficacy was tested by coagulation in tube. It showed that the goat anti-rabbit IgG had been labeled by fluorescence isothiocyanate (FITC). The results showed that positive reactions were 100% for the large yellow croakerPseudosciaena crocea with typical symptom of vibrio infection

while the positive reaction to the pathogen in healthy yellow croakers reached 40%

but seemed negative for aquaculture water. The results demonstrated that this fast and indirect fluorescent antibody assay can be used not only to test the vibrio pathogen in diseased yellow croaker but also in infected animals with no symptom.

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references

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