Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits inFenneropenaeus chinensis

Yue Zhiqin; Wang Weiji; Kong Jie; Dai Jixun

doi:10.1007/BF02842689

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Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits inFenneropenaeus chinensis

Updated：2023-05-16

- Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits inFenneropenaeus chinensis
- Journal of Oceanology and Limnology Vol. 23, Issue 4, Pages: 442-447(2005)
- Affiliations：
  
  1. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Yellow Sea Fisheries Research Institute
  2. College of Marine Life Science Ocean University of China
  3. Food Laboratory of qingdao Entry-Exit Inspection and Quarantine Bureau
- Author bio：
- Funds：
- DOI：10.1007/BF02842689
  CLC：
- Published：2005
- Accepted：
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Zhiqin, Y., Weiji, W., Jie, K. et al. Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits inFenneropenaeus chinensis, ., Chin. J. Ocean. Limnol. 23, 442–447 (2005). https://doi.org/10.1007/BF02842689
DOI：

Zhiqin, Y., Weiji, W., Jie, K. et al. Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits inFenneropenaeus chinensis, ., Chin. J. Ocean. Limnol. 23, 442–447 (2005). https://doi.org/10.1007/BF02842689 DOI：

摘要

Abstract

Amplified fragment length polymorphism (AFLP) technique was used to analyze the fingerprinting of four successive generations ofFenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection

which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to further characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels

re-amplified

cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS (database of Genome Sequence Survey); and the BLAST analysis showed low similarity to the function genes

indicating these markers were tightly linked to a disease-resistance trait but were not functional genes.

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references

Agresti, J. J., S. Seki, A. Cnaani, S. Poompuang, E. M. Hallerman, N. Umiel, G. Hulata, G. A. E. Gall, and B. May, 2000. Breeding new strains of tilapia: development of an artificial center of origin and linkage map based on AFLP and microsatellite loci.Aquac. 185: 43–56.

Alonso-Blanco, C., A. J. M. Peeters, M. Koornneef, C. Lister, C. Dean, N. van den Bosch, J. Pot, and M. T. R. Kuiper, 1998. Development of an AFLP based linkage map of Ler, Col, and CviArabidopsis thalianan ecotypes and construction of a Ler/Cvi recombinant inbred line population.Plant J. 14: 259–271.

Becker, J., P. Vos, M. Juiper, F. Salamini, and M. Heun, 1995. Combined mapping of AFLP and RFLP markers in barley.Mol. Gen. Genet. 249: 65–73.

Cho, Y. G., M. W. Blair, O. Panaud, and S. R. McCouch, 1996. Cloning and mapping of variety-specific rice genomic DNA sequences amplified fragment length polymorphisms (AFLP) from silver-stained polyacrylamide gels.Genome 39: 373–378.

Congiu, L., I. Dupanloup, T. Patarnello, F. Fontana, R. Rossi, G. Arlati, and L. Zane, 2001. Identification of interspecific hybrids by amplified fragment length polymorphism: the case of sturgeon.Mol. Ecol. 10: 2355–2359.

Deng, J. Y., C. C. Ye, and Y. C. Liu, 1990.Penaeus chinensis in the Bohai and Yellow Seas-its Biology and Management. Ocean Press, Beijing, China, p. 36–164. (in Chinese)

English, L. J., J. A. Nell, G. B. Maguire, and R. D. Ward, 2001. Allozyme variation in the three generations of whole weight in Sydney rock oysters (Saccostrea golmerata).Aquac. 193: 213–225.

Jin, H., L. Domier, X. Shen, and F. Kolb, 2000. Combined AFLP and RFLP mapping in two hexaploid oat recombinant inbred populations.Genome 43: 94–101.

Knorr, C., H. H. Cheng, and J. B. Dodgson, 2001. DNA cloning and sequence analysis of chicken AFLP.Animal Genetics 32: 156–159.

Kusumo, H. T., L. D. Druchl, 2000. Genetic structure variability of kelpAlaria marginata over space and time.J. Phycol. 36: 39–40.

Liu, Z., A. Nichols, P. Li, and R. A. Dunham, 1998. Inheritance and usefulness of AFLP markers in channel catfish (Ictalurus punctatus), blue catfish (I. Frucatus), and their F1, F2, and backcross hybrids.Mol. Gen. Genet. 258: 260–268.

Moore, S. S., V. Whan, G. P. Davis, K. Byrne, D. J. S. Hetzel and N. Preston, 1999. The development and application of genetic markers for the Kuruma prawnPenaeus japonicus.Aquac. 173: 19–32.

Negi, M. S., M. Devic, M. Delseny, and M. Lakshmikumaran, 2000. Identification of AFLP fragments linked to seed coat colour inBrassica juncea and conversion to a SCAR marker for rapid selection.Theor. Appl. Genet. 101: 146–152.

Saliba-Colombani, V., M. Causse, L. Gervais, and J. Philouze, 2000. Efficiency of RFLP, RAPD, and AFLP markers for the constructions of an intraspecific map of the tomato genome.Genome 43: 29–40.

Sambrook, J., E. F. Fritsch and T. Maniatis, 1989. Molecular Cloning. A Laboratory Manual. Cold Springs Harbor Laboratory Press.

Terauchi, R., and G. Kahl, 1999. Mapping of the Sioscorea tokoro genome: AFLP markers linked to sex.Genome 45: 752–762.

Vos, P., R. Hodgers, M. Bleeker, M. Reijans, T. van de Lee, M. Hornes, A. Frijters, J. Pot, J. Peleman, M. Kuiper and M. Zabeau, 1995. AFLP: a new technique for DNA fingerprinting.Nucl. Acids Res. 23: 4407–4414.

Wilson, K., Y. Li, V. Whan, S. Lehnert, K. Byrne, S. Moore, S. Pongsomboon, A. Tassanakajon, G. Rosenberg, E. Ballment, Z. Fayazi, J. Swan, M. Kenway, and J. Benzie, 2002. Genetic mapping of the black tiger shrimpPenaeus monodon with amplified fragment length polymorphism.Aquac. 204: 297–309.

Zhang, Q. W., P. Liu, W. J. Wang, J. Kong, and Q. Y. Wang, 2002. A preliminary study on genetic selection for disease resistant strain inFenneropenaeus chinensis culture.Marine Fisheries Research 23(2): 53–57. (in Chinese)

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