Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization

Zhan Wen-bin; Wang Yuan-hong; Zhang Zhi-dong; Fukuda Hideo

doi:10.1007/BF02842670

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Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization

Updated：2023-05-16

- Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization
- Journal of Oceanology and Limnology Vol. 18, Issue 3, Pages: 241-246(2000)
- Affiliations：
  
  1. Aquaculture Research Laboratory Ocean University of Qingdao
  2. Tokyo University of Fisheries
- Author bio：
- Funds：
- DOI：10.1007/BF02842670
  CLC：
- Published：2000
- Accepted：
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Wen-bin, Z., Yuan-hong, W., Zhi-dong, Z. et al. Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization., Chin. J. Ocean. Limnol. 18, 241–246 (2000). https://doi.org/10.1007/BF02842670
DOI：

Wen-bin, Z., Yuan-hong, W., Zhi-dong, Z. et al. Detection of white spot syndrome virus (WSSV) ofPenaeus chinensis by in situ hybridization., Chin. J. Ocean. Limnol. 18, 241–246 (2000). https://doi.org/10.1007/BF02842670 DOI：

摘要

Abstract

White Spot Syndrome Virus (WSSV) was purified from hemolymph of infected shrimp. After nucleic acid extraction from the purified virus particles

EcoR I-digested fragments of the WSSV genome were cloned; three of these fragments were used as non-radioactive probes labeled with DIG-11-dUTP. The probes hybridized in situ

with sections located in the nuclei of all WSSV-infected tissues. The virus was detected in the gill

stomach

epidermis

and connective tissue and so on

but not detected in healthy shrimp tissues and epithelial cells of hepatopancreatic tubules of diseased shrimp.

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references

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