The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallopChlamys farreri

Liu Baozhong; You Feng; Dong Bo; Xiang Jianhai

doi:10.1007/BF02842631

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The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallopChlamys farreri

Updated：2023-05-16

- The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallopChlamys farreri
- Journal of Oceanology and Limnology Vol. 24, Issue 3, Pages: 295-299(2006)
- Affiliations：
  
  Institute of Oceanology Chinese Academy of Sciences
- Author bio：
- Funds：
- DOI：10.1007/BF02842631
  CLC：
- Published：2006
- Accepted：
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Baozhong, L., Feng, Y., Bo, D. et al. The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallopChlamys farreri, ., Chin. J. Ocean. Limnol. 24, 295–299 (2006). https://doi.org/10.1007/BF02842631
DOI：

Baozhong, L., Feng, Y., Bo, D. et al. The comparison between allozyme and RAPD makers for the population genetic structure analysis of scallopChlamys farreri, ., Chin. J. Ocean. Limnol. 24, 295–299 (2006). https://doi.org/10.1007/BF02842631 DOI：

摘要

Abstract

To compare genetic markers for population genetics analysis

allozyme electrophoresis and random amplified polymorphic DNA (RAPD) were used to detect the genetic structure of scallopChlamys farreri population. Thirteen enzymes (MDH

IDH

GPI

PGM

PEP−LG

PEP−PP

ACP

AAT

SOD

EST) in three buffer systems (TC

Ph6.9; TMME

Ph 7.4; and EBT

pH8.9) were selected and 22 loci were used for the analysis

among them 7 loci (Gpi

Pgm

Pep−LG-1

Pep−PP Aat-2

Est-2

Est-3) were polymorphic which attributed 31.82% to the total. The average of heterozygosity was 0.113 and most of the studied loci showed heterozygote deficiencies. The same specimens were investigated using 10 arbitrarily selected primers (10-base). Twenty two of 54 RAPD fragments were polymorphic with average heterozygosity of 0.194. The result indicated that the two types of markers reflected a consistent trend in the parameter values of genetic diversity of the population

but RAPD revealed more information of genetic variation than allozyme electrophoresis.

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references

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